ID   SYL_AZOSB               Reviewed;         873 AA.
AC   A1KAH5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=azo3215;
OS   Azoarcus sp. (strain BH72).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC   Azoarcus.
OX   NCBI_TaxID=418699;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BH72;
RX   PubMed=17057704; DOI=10.1038/nbt1243;
RA   Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J.,
RA   Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C.,
RA   Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J.,
RA   Weidner S., Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.;
RT   "Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp.
RT   strain BH72.";
RL   Nat. Biotechnol. 24:1385-1391(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; AM406670; CAL95831.1; -; Genomic_DNA.
DR   RefSeq; WP_011766939.1; NC_008702.1.
DR   AlphaFoldDB; A1KAH5; -.
DR   SMR; A1KAH5; -.
DR   STRING; 62928.azo3215; -.
DR   PRIDE; A1KAH5; -.
DR   EnsemblBacteria; CAL95831; CAL95831; azo3215.
DR   KEGG; aoa:dqs_3352; -.
DR   KEGG; azo:azo3215; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_4; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000002588; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 2.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..873
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009290"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           628..632
FT                   /note="'KMSKS' region"
FT   BINDING         631
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   873 AA;  96943 MW;  9516D7967993F4CB CRC64;
     MQDKYQPAAV ETAAQQHWES TAAFRVTEDA TRPKYYCLSM FPYPSGKLHM GHVRNYTIGD
     VLARFHRMRG YNVLQPMGWD AFGMPAENAA IQNNVPPAKW TYANIDYMKG QLKRLGFAID
     WSREVATCTP EYYRWEQWLF TRLFEKGLIY KKLGTVNWDP VDETVLANEQ VIDGRGWRSG
     ALVEKREIPM YYMKITAYAD ELLEALDGLP GWPEQVKLMQ KNWIGRSEGV EVHFPYDLST
     IGSSGVLKVF TTRGDTLMGA TYVAVAAEHP LATQAAAGNP ELAAFIDECK QGGVAEADLA
     TMEKKGMPTG LRVVHPITGE YLPVWVANYV LMGYGEGAVM AVPAHDERDF AFATKYKLPI
     KMVVRSTHDA YENVAAPWID AYAEHGKLVN SGKYDNLHFQ DAVDAIAADL AAKGLGNKRV
     QYRLRDWGIS RQRYWGCPIP MVRCDDCGDV PVPDEQLPVV LPENVEITGR GSPLAKMPEF
     YQCSCPKCGK PARRETDTMD TFVESSWYFL RYASPDSTTA MVDERVNYWA PVDQYIGGIE
     HAILHLLYSR FFTRAMRDCG LVNVSEPFTN LLTQGMVVAE TYYRELDGGK KQWLNPADVQ
     VERDERGRIT AAKLASDGLP VVIGGTEKMS KSKNNGVDPQ ALVDQYGADT ARLFIIFAAP
     PDQQLEWSDS GVEGASRFLR RVWNFGHAFA SEFRAALPAA RALAAGTLPA ALADVRREIH
     THLKQANYDF GKHQFNTVVS AAMKILNALE KAPRDDAAAH AEVAEEGFSI LVRLLSPITP
     HIAHALWQDC GFGGDIVQAA WPEPLEAALK QDEIELMLQV NGKLRGSVKV AADAGKSDIE
     ALALASEAAQ KFMEGKPPKK VVVVPGRLVN IVV