ID   SYL_BACFN               Reviewed;         943 AA.
AC   Q5L7B4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-JUN-2005, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=BF4372;
OS   Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019
OS   / NCTC 9343 / Onslow).
OC   Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC   Bacteroides.
OX   NCBI_TaxID=272559;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow;
RX   PubMed=15746427; DOI=10.1126/science.1107008;
RA   Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V.,
RA   Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A.,
RA   Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A.,
RA   Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J.,
RA   Barrell B.G., Parkhill J.;
RT   "Extensive DNA inversions in the B. fragilis genome control variable gene
RT   expression.";
RL   Science 307:1463-1465(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CR626927; CAH10038.1; -; Genomic_DNA.
DR   RefSeq; WP_010993827.1; NC_003228.3.
DR   AlphaFoldDB; Q5L7B4; -.
DR   SMR; Q5L7B4; -.
DR   STRING; 272559.BF9343_4257; -.
DR   EnsemblBacteria; CAH10038; CAH10038; BF9343_4257.
DR   KEGG; bfs:BF9343_4257; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_10; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000006731; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 3.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..943
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009293"
FT   MOTIF           40..51
FT                   /note="'HIGH' region"
FT   MOTIF           717..721
FT                   /note="'KMSKS' region"
FT   BINDING         720
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   943 AA;  107868 MW;  50A0499C6FD5A16C CRC64;
     MEYNFREIEK KWQKIWVDNH TYQVNEDASK QKFYVLNMFP YPSGAGLHVG HPLGYIASDI
     YARYKRLQGF NVLNPMGYDA YGLPAEQYAI QTGQHPAITT VNNINRYREQ LDKIGFSFDW
     NREIRTCDPE YYHWTQWAFI KMFNSYYCND EKQARPIEEL IEAFSTNGTQ GMNVACGEEM
     DFTADEWNAK SEKEQQEILM NYRIAYLGNT MVNWCPALGT VLANDEVVDG VSERGGYPVI
     QKVMRQWCLR VSAYAQRLLD GLETVEWTDS LKETQRNWIG RSEGAEMNFK VKDSDIEFTI
     FTTRADTVFG VTFMVLAPES ELVAKLTTPE QKAEVDAYLD RTKKRTERER IADRSVSGVF
     SGSYAINPLT NEPIPVWISD YVLAGYGTGA IMAVPAHDSR DYAFAKHFNL EIRPLIEGCD
     VSEESFDAKE GIMMNSPRPG APEGGLVLNG LTVKEAIAKT KEYIKATGLG RVKVNFRLRD
     AIFSRQRYWG EPFPVYYKDG MPYMIDESCL PLELPEVAKF LPTETGEPPL GHATKWAWDT
     VNKCVTDNEN IDNITIFPLE LNTMPGFAGS SAYYLRYMDP RNHEALVSPA VDQYWKNVDL
     YVGGTEHATG HLIYSRFWNK FLHDWGISVA EEPFQKLVNQ GMIQGRSNFV YRIKDTNTFV
     SLNLKDQYEV TPIHVDVNIV SNDILDLEAF KAWRPEYETA EFILEDGKYI CGWAVEKMSK
     SMFNVVNPDM IVEKYGADTL RMYEMFLGPV EQSKPWDTNG IDGVHRFIKK FWSLFYDRNG
     EYLVKDEPAT KEELKALHKL IKKVTGDIEQ FSYNTSVSAF MICVNELSSL KCNKKEVLEQ
     LIVVLAPFAP HVCEELWDTL GSTTSVCDAQ WPAFNEQYLV EDTVNYTISF NGKARFNMEF
     PANAASDAIQ ATVLADERSL KWTEGKTPKK VIVVPKKIVN IVI