SYL_BRASB
ID SYL_BRASB Reviewed; 874 AA.
AC A5E8H4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=BBta_0171;
OS Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium.
OX NCBI_TaxID=288000;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BTAi1 / ATCC BAA-1182;
RX PubMed=17540897; DOI=10.1126/science.1139548;
RA Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C.,
RA Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E.,
RA Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL Science 316:1307-1312(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; CP000494; ABQ32468.1; -; Genomic_DNA.
DR RefSeq; WP_011942689.1; NC_009485.1.
DR AlphaFoldDB; A5E8H4; -.
DR SMR; A5E8H4; -.
DR STRING; 288000.BBta_0171; -.
DR EnsemblBacteria; ABQ32468; ABQ32468; BBta_0171.
DR KEGG; bbt:BBta_0171; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_5; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000000246; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..874
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000009300"
FT MOTIF 43..53
FT /note="'HIGH' region"
FT MOTIF 630..634
FT /note="'KMSKS' region"
FT BINDING 633
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 874 AA; 97492 MW; 5AA7D708047FC63D CRC64;
MTSERYNARE AEPRWQRQWD EKAIFATKND DPRPKYYVLE MFPYPSGRIH IGHVRNYTLG
DVLARFMRAK GFNVLHPMGW DAFGLPAENA AIERKVAPKA WTYDNIAAMK KQLRSIGLSL
DWAREFATCD PSYYKHQQKM FLDFMQAGLV EREQRKVNWD PVDMTVLANE QVIDGRGWRS
GAVVEQREMN QWVFKITKYS QELLDALDGL DRWPDKVRLM QRNWIGRSEG LLIRFALDPQ
TTPAGETELK IFTTRPDTLF GAKFMAISAD HPLAQAAAAK NPELAAFIAE IKRIGTAQEA
IDTAEKQGFD TGIRAVHPFD PSWTLPVYVA NFVLMEYGTG AIFGCPAHDQ RDLDFVNKYG
LGNTPVVCPE GQDPASFVIT DTAFDGDGRL INSRFLDGMT IAQAKDEVAK RLEAEQRGGA
AVGERQVNFR LRDWGISRQR YWGCPIPVIH CPTCDVVPVP AKDLPVVLPD DVTFDKPGNA
LDHHPTWKHV TCPKCGGKAT RETDTMDTFV DSSWYFARFT DPWNETAPTT PDVANRMMPV
DQYIGGVEHA ILHLLYSRFF TRAMKATGHV AMDEPFAGMF TQGMVVHETY QKADGTYVNP
AEVKIEVGGN GRRAVLTATG EDITIGPIEK MSKSKKNTVD PDDIIESYGA DVARWFMLSD
SPPDRDVIWS DERVQGAARF VQRLWRLVNE SVAAGQEAPS SRPATFGPDA LALRKAAHGA
LDKVTGGIER LHFNVCLAHI REFANALAEV LARPAKPAPD LAWAIREAAQ ILVQLFSPMM
PHLAEECWQV LGQGGLISEA SWPQIERDLL VEDSVTLVVQ VNGKKRGEVT VASNAQNPEI
ESAVLALDAV KLALDGKPVR KVIIVPKRIV NVVG