SYL_BRASO
ID SYL_BRASO Reviewed; 874 AA.
AC A4YJS8;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=BRADO0189;
OS Bradyrhizobium sp. (strain ORS 278).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium.
OX NCBI_TaxID=114615;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ORS 278;
RX PubMed=17540897; DOI=10.1126/science.1139548;
RA Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C.,
RA Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E.,
RA Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL Science 316:1307-1312(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; CU234118; CAL74154.1; -; Genomic_DNA.
DR RefSeq; WP_011923445.1; NC_009445.1.
DR AlphaFoldDB; A4YJS8; -.
DR SMR; A4YJS8; -.
DR STRING; 114615.BRADO0189; -.
DR EnsemblBacteria; CAL74154; CAL74154; BRADO0189.
DR KEGG; bra:BRADO0189; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_5; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR BioCyc; BSP114615:BRADO_RS00905-MON; -.
DR Proteomes; UP000001994; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..874
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000009301"
FT MOTIF 43..53
FT /note="'HIGH' region"
FT MOTIF 630..634
FT /note="'KMSKS' region"
FT BINDING 633
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 874 AA; 97349 MW; BE0C6EF60A3DCA47 CRC64;
MTSERYNARE AEPRWQRQWD EKAIFATKND DPRPKYYVLE MFPYPSGRIH IGHVRNYTLG
DVLARFMRAK GFNVLHPMGW DAFGLPAENA AIERKVAPKA WTYDNIAAMK KQLRSIGLSL
DWAREFATCD PSYYKHQQKM FLDFMQAGLV EREQRKVNWD PVDMTVLANE QVIDGRGWRS
GAVVEQREMN QWVFKITKYS QELLDALDGL DRWPDKVRLM QRNWIGRSEG LLIRFALDPK
TVPAGESELK IFTTRPDTLF GAKFMAISAD HPLAQAAAAK NPELAAFIAE IKRIGTAQEA
IDTAEKQGFD TGIRAVHPFD PSWTLPVYVA NFVLMEYGTG AIFGCPAHDQ RDLDFVNKYG
LGNTPVVCPE GQDPASFVIT DTAYDGDGRM INSRFLDGMT IAQAKDEVAK RLESEQRGGS
PIGERQVNFR LRDWGISRQR YWGCPIPVIH CATCDVVPVP AGDLPVVLPD DVTFDKPGNA
LDHHPTWKHV TCPKCGGKAT RETDTMDTFV DSSWYFARFT DPWNETAPTT PAVANRMMPV
DQYIGGVEHA ILHLLYSRFF TRAMKATGHV AMDEPFAGMF TQGMVVHETY QKADGTYVNP
ADVKIEVGGN GRRAVLTATG EDITIGPIEK MSKSKKNTVD PDDIIESYGA DVARWFMLSD
SPPDRDVIWS DERVQGAARF VQRLWRLVNE SVAASAEAPS SRPANFSADA LALRKAAHGA
LDKVTGGIER LHFNVCLAHI REFANALADV LAKPAKPAPD LAWATREAAG ILVQLFSPMM
PHLAEECWQV LGHSGLISEA SWPQIERDLL VEDSMTLVVQ VNGKKRGEVT VASNAQNPEI
ESAVLALDAV KVALDGKPVR KVIIVPKRIV NVVG
