SYL_CALS4
ID SYL_CALS4 Reviewed; 819 AA.
AC Q8RCT6;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=TTE0324;
OS Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM
OS 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Caldanaerobacter.
OX NCBI_TaxID=273068;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15242 / JCM 11007 / NBRC 100824 / MB4;
RX PubMed=11997336; DOI=10.1101/gr.219302;
RA Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y.,
RA Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R.,
RA Wang J., Yu J., Yang H.;
RT "A complete sequence of the T. tengcongensis genome.";
RL Genome Res. 12:689-700(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; AE008691; AAM23616.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8RCT6; -.
DR SMR; Q8RCT6; -.
DR STRING; 273068.TTE0324; -.
DR EnsemblBacteria; AAM23616; AAM23616; TTE0324.
DR KEGG; tte:TTE0324; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_9; -.
DR OMA; TFMVLAP; -.
DR Proteomes; UP000000555; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 2.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..819
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000152107"
FT MOTIF 42..53
FT /note="'HIGH' region"
FT MOTIF 578..582
FT /note="'KMSKS' region"
FT BINDING 581
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 819 AA; 95501 MW; 7D9CB46702513E83 CRC64;
MEDMAYSVEI DRKWQKIWEE TKLYKFNPEN VDKKLYCLEM FPYPSGAKLH IGHWYNYGPT
DSWARMKRMQ GYEVFHPMGF DAFGLPAENY AIKTGIHPYD STMENIRTME RQLREMGVTF
DWDYEIITCL PEYYKWTQWI FLKLYEAGLA YRKKAPVNWC PSCQTVLANE QVIDGKCERC
GTEVTKKELT QWFFKITAYA EELLEKLDDL DWPEKTKMMQ RNWIGKSEGA EIEFKIDGKD
LSFRVFTTRA DTLFGATYVV LAPEHELVDL ITTEEYKEAV EEYKEYAKKQ SEIERLSTEK
EKTGVFTGAY AIHPLTGEKL PIWIADYVLV TYGTGCVMGV PGHDERDYEF ATKYNLPIKR
VIKGVGDVDD SLPFVEYGIL VNSEEFTGMT SEEARVKIVE KLKAEGKAEF KVNYRMRDWL
VSRQRYWGAP IPIIHCERCG IVPVPEEDLP VLLPYDVEFE PTGESPLKKH AGFMNVTCPK
CGGPALRDPD TLDTFVDSSW YYLRYPDNKN DKEPFNKEWI NKMLPVDKYV GGAEHATMHL
LYSRFITKVL RDLGYLNFDE PFLSLVHQGT ILGPDGSRMS KSRGNVISPD DYIKQYGSDV
FRLYLMFGFS YSEGGPWSDE GIKAIARFVN RVERFIEKFI ETRQNPGKTK EEMEDAEKEL
NYVRHYTIKH VTLDADKFEF NTAIARIMEL VNALYKYENE VEVKNMKFYE DVVRDFVKIL
APFAPHFSEE MWERLGYEYS VFNQKWPEWD EKALERDMIE IAIQVNGKVR SKAQVPSNAT
DEELKQIALS DERVKSYLDG KEIKKVIIVK NRLVNIVVN
