ID   SYL_CHLCV               Reviewed;         820 AA.
AC   Q822R7;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=CCA_00612;
OS   Chlamydia caviae (strain ATCC VR-813 / DSM 19441 / 03DC25 / GPIC)
OS   (Chlamydophila caviae).
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC   Chlamydia/Chlamydophila group; Chlamydia.
OX   NCBI_TaxID=227941;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-813 / DSM 19441 / 03DC25 / GPIC;
RX   PubMed=12682364; DOI=10.1093/nar/gkg321;
RA   Read T.D., Myers G.S.A., Brunham R.C., Nelson W.C., Paulsen I.T.,
RA   Heidelberg J.F., Holtzapple E.K., Khouri H.M., Federova N.B., Carty H.A.,
RA   Umayam L.A., Haft D.H., Peterson J.D., Beanan M.J., White O.,
RA   Salzberg S.L., Hsia R.-C., McClarty G., Rank R.G., Bavoil P.M.,
RA   Fraser C.M.;
RT   "Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC):
RT   examining the role of niche-specific genes in the evolution of the
RT   Chlamydiaceae.";
RL   Nucleic Acids Res. 31:2134-2147(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; AE015925; AAP05354.1; -; Genomic_DNA.
DR   RefSeq; WP_011006569.1; NC_003361.3.
DR   AlphaFoldDB; Q822R7; -.
DR   SMR; Q822R7; -.
DR   STRING; 227941.CCA_00612; -.
DR   EnsemblBacteria; AAP05354; AAP05354; CCA_00612.
DR   KEGG; cca:CCA_00612; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_0; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000002193; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..820
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000151996"
FT   MOTIF           40..51
FT                   /note="'HIGH' region"
FT   MOTIF           601..605
FT                   /note="'KMSKS' region"
FT   BINDING         604
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   820 AA;  93854 MW;  47DBBF2B175B10E8 CRC64;
     MRYDPSLIEK KWQEFWKEHK SFEADEASDK PKYYVLDMFP YPSGAGLHVG HLIGYTATDI
     VARYKRAKGY AVLHPMGWDS FGLPAEQYAV RTGTHPRETT QKNIENFRKQ LSAMGFSYDE
     GREFATSDPD YYRWTQKLFL FLYEKGLAYM ADMAVNYCPE LGTVLSNEEV ENGLSVEGGY
     PVERRMLRQW ILRITAYSDQ LLEGLEDLDW PENVKQLQRN WIGKSEGALV RFEVNNERFL
     EVFTTRPDTI GGVSFLVVAP EHPEVNRLIS ENQREAVESY IRAAQSKSER DRISETKVKT
     GVFTGTYAKH PVTGADIPIW ISDYVILGYG SGVVMGVPAH DERDREFAEA FSLPIYEVLD
     KDECCIHSNH GDFLLDGLAG QEARDYVIAY LQKKNLGEAK VAYKLRDWLF SRQRYWGEPI
     PIIHFEDGTC RPLEDDELPL LPPEIQDYRP EGFGQGPLAK VKEWVDIHDT KTNRRGRRET
     HTMPQWAGSC WYYLRFCDAH NSQAPWSNEN ERYWMPVDLY IGGAEHAVLH LLYSRFWHRV
     FYEAGMVSTA EPFKKLINQG LVLATSYRIP GKGYVYPEDA REDNGVWMSA SGEELEVRQE
     KMSKSKLNGV DPQILIDEFG ADALRMYAMF SGPLDKNKLW SNQGVSGCRR FLNRFYEMAT
     SASVQDVDDP KGMALAHRLV HRVGEDIEKM SLNTIPSSFM EFINEFVKLD IYPRSALAMV
     VQALAPIAPH ISEELWTVLG YAPGIDAAGW PKVDPKYLED TSVTFVIQVN GKLRARLDMD
     KATSKEDVLS LAREAVAKYL EGKEVKKEVF VPNRLVNFVL