ID   SYL_CHLFF               Reviewed;         820 AA.
AC   Q254X5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=CF0391;
OS   Chlamydia felis (strain Fe/C-56) (Chlamydophila felis).
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC   Chlamydia/Chlamydophila group; Chlamydia.
OX   NCBI_TaxID=264202;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fe/C-56;
RX   PubMed=16766509; DOI=10.1093/dnares/dsi027;
RA   Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H.,
RA   Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H.,
RA   Hattori M., Kuhara S., Shirai M.;
RT   "Genome sequence of the cat pathogen, Chlamydophila felis.";
RL   DNA Res. 13:15-23(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; AP006861; BAE81163.1; -; Genomic_DNA.
DR   RefSeq; WP_011457943.1; NC_007899.1.
DR   AlphaFoldDB; Q254X5; -.
DR   SMR; Q254X5; -.
DR   STRING; 264202.CF0391; -.
DR   KEGG; cfe:CF0391; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_0; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000001260; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..820
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009321"
FT   MOTIF           40..51
FT                   /note="'HIGH' region"
FT   MOTIF           601..605
FT                   /note="'KMSKS' region"
FT   BINDING         604
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   820 AA;  94247 MW;  EAC547EDE26B1A2C CRC64;
     MRYDPSLIEK KWQEFWKEHK SFKANEADDK PKYYVLDMFP YPSGAGLHVG HLIGYTATDI
     VARYKRAKGF SVLHPMGWDS FGLPAEQYAV RTGTHPRETT QKNIENFRKQ LSAMGFSYDE
     GREFATSDPE YYRWTQKLFL LLYEKGLAYM ADMAVNYCPE LGTVLSNEEV ENGLSIEGGY
     PVERRMLRQW ILRITAYSDQ LLEGLEGLDW PENVKQLQRN WIGKSEGALV RFKVNNQNFL
     EVFTTRPDTL CGVSFLVIAP EHPEVGQLIA EDRREEVEAY ICRAQSKSER DRISESKIKS
     GVFTGTYAKH PITGADIPIW VSDYVILGYG SGVVMGVPAH DERDREFAET FSLPIYEVLD
     TDGYCIHSNH GDFLLDGLWG REAQDYVIAY LQKKNLGESK VAYKLRDWLF SRQRYWGEPI
     PIIHFEDGTC RPLEDDELPL LPPEIQDYRP EGFGQGPLAK VKEWVDIHDK KTNRQGRRET
     HTMPQWAGSC WYYLRFCDAL NSQAPWSNEK EKYWMPVDLY IGGAEHAVLH LLYSRFWHRV
     FYDAGMVSTP EPFKKLINQG LVLATSYRIP GKGYVYPEDA HEDNGVWMST SGEELEVRQE
     KMSKSKLNGV DPQILIEEFG ADALRMYAMF SGPLDKNKLW CNQGVSGCRR FLNRFYELVT
     SSLVQDIDDP KGMYLAHRLV HRVGEDIEKM SLNTIPSSFM EFINEFVKLD IYPKSALAMV
     VRALAPIAPH ISEELWTILG NAPGIDQAGW PEVDPKYLED TSVTFVIQVN GKLRARLDIS
     KNAAREEVLS LAREAVSRYL EDKEVKKEIF VPNRLVNFVL