SYL_CHLL3
ID SYL_CHLL3 Reviewed; 805 AA.
AC Q3B2E1;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Plut_1636;
OS Chlorobium luteolum (strain DSM 273 / BCRC 81028 / 2530) (Pelodictyon
OS luteolum).
OC Bacteria; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae;
OC Chlorobium/Pelodictyon group; Pelodictyon.
OX NCBI_TaxID=319225;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 273 / BCRC 81028 / 2530;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F.,
RA Land M., Kyrpides N., Ivanova N., Richardson P.;
RT "Complete sequence of Pelodictyon luteolum DSM 273.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; CP000096; ABB24490.1; -; Genomic_DNA.
DR RefSeq; WP_011358362.1; NC_007512.1.
DR AlphaFoldDB; Q3B2E1; -.
DR SMR; Q3B2E1; -.
DR STRING; 319225.Plut_1636; -.
DR EnsemblBacteria; ABB24490; ABB24490; Plut_1636.
DR KEGG; plt:Plut_1636; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_10; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000002709; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 2.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..805
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000009388"
FT MOTIF 40..51
FT /note="'HIGH' region"
FT MOTIF 576..580
FT /note="'KMSKS' region"
FT BINDING 579
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 805 AA; 91936 MW; C5D33F532F3DD313 CRC64;
MKYDFSHTEK KWQAYWQEHG TFQAEEGQEK PKYYVLDMFP YPSGSGLHVG HLEGYTASDI
VARYRRSRGN NVLHPMGWDA FGLPAEQYAI KTGTHPSLTT ENNIRSFRET LQAMGFSYDW
SREINTTDPG YFRWTQWIFL KLYEMGLAYQ SEVDVNWCVE LRAVLANEEV EEKIAEGLTV
VRRPLRQWVL KITAYAERLL LDLDGLDWPE NVKQMQRNWI GRSEGVEVDF ELRCHRKMLR
VYTTRPDTLF GATYLVISPE HPMAEKLATA PQLVEVKNYI SKAKLKTELE RTGLQKDKTG
VFTGSYAINP ANGEALPVWI SDFVLTSYGT GAIMSVPAHD SRDWEFAKKF GLPIVEVVKS
PHDVQDAVYE GKDSTAVNSS NSEISLDGLA FPEAFERMAS WLELKKCGER KVNYRLRDWI
FSRQRYWGEP IPVKHYEDGS LGTETDLPLR LPEVEAYQPT ETGESPLANI PEWLYGTDGK
GAFRRETNTM PQWAGSCWYY LRFIDPRNSE HLVDAEKEHY WMNVDLYIGG AEHAVLHLLY
ARFWHKVLYD LKVVSTKEPF QKLFNQGMIL GEDGEKMSKS RGNVIPADHV LEKYGADAVR
LYEMFLGPLE QVKPWNTNGI EGVSRFLNRV WRLVHPDTEG PAAVLDEAAM PGELLRRMHK
TVKKVREDTE CLKFNTAIAE MMVFVNDLHR TGNRNREAIE TLVLLLAPYA PHISEELWEA
LGHPESIARA PFPTFDPALA ANDVLTIAVQ VNGKLRGTFE AAKDASKEEM LEAARAVESV
RKFIEGSTVV KEIVVPGKLI NFAVK
