ID   SYL_CHLPD               Reviewed;         816 AA.
AC   A1BDY0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   OrderedLocusNames=Cpha266_0551;
OS   Chlorobium phaeobacteroides (strain DSM 266).
OC   Bacteria; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae;
OC   Chlorobium/Pelodictyon group; Chlorobium.
OX   NCBI_TaxID=290317;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 266;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T.,
RA   Overmann J., Bryant D.A., Richardson P.;
RT   "Complete sequence of Chlorobium phaeobacteroides DSM 266.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000492; ABL64607.1; -; Genomic_DNA.
DR   RefSeq; WP_011744440.1; NC_008639.1.
DR   AlphaFoldDB; A1BDY0; -.
DR   SMR; A1BDY0; -.
DR   STRING; 290317.Cpha266_0551; -.
DR   EnsemblBacteria; ABL64607; ABL64607; Cpha266_0551.
DR   KEGG; cph:Cpha266_0551; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_10; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000008701; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 2.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..816
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009322"
FT   MOTIF           40..51
FT                   /note="'HIGH' region"
FT   MOTIF           576..580
FT                   /note="'KMSKS' region"
FT   BINDING         579
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   816 AA;  93335 MW;  6770B1DAC50560F1 CRC64;
     MRYDFSTIEK KWQSFWLKNN SFSSGESTDK PKYYVLDMFP YPSGSGLHVG HLEGYTATDI
     VARYKRSCGY NVLHPMGWDA FGLPAEQFAI KTGTHPKNTT EKNISSFRET LQAMGFSYDW
     SREINTTSPD YFRWTQWIFI KLYELGLAYV SEVDINWCEE LKTVLANEEV EEKVKEGYTV
     IRKPLRQWVL KITAYAERLL DDLDELDWPE NVKQMQRNWI GRSEGMEIDF ELRCHNRKLR
     VYTTRPDTLF GATFLVISPE HPLALKLATA QQLVAVTQYI KEAKLKSELE RTGLQKDKTG
     IFTGSFAINP ATGDPLPVWI SDFVLTSYGT GAIMSVPAHD SRDWEFAKKF GLPIIEVIKS
     PDGVDDAAFE GKESVSINSS NPEISIDGLH FSEAFQIMAH WLETKGLGER KVTYKLRDWI
     FSRQRYWGEP IPVKHYEDGS IRTESNLPLQ LPEVEAYQPS ETGESPLATM HDWLYGADEF
     GSFRRETNTM PQWAGSCWYY LRFIDPENNA QLIDPAKEKY WMNVDLYIGG AEHAVLHLLY
     ARFWHKVLYD LGVVSSREPF KKLFNQGMIL GEDNEKMSKS RGNVIPADQV LKDYGADAVR
     LYEMFLGPLE QVKPWNTNGI EGISRFLGKV WRLVYPDSEN PDQRDQAAVL DENPLPEILQ
     RRMHKTIKKV TEDTDHLKFN TAISEMMVFV NELQKNGCRQ KSAVENLLLM LAPYAPHITA
     ELWQAIGHTS PISAEPFPVF DPAIAEDPVV TIAVQVNGKL RGRFTAPAKS PEESLVDMAK
     HVDTVIKFLE GQTIVKEIVI QDKLVNFAVK QSIPNH