SYL_CHLPM
ID SYL_CHLPM Reviewed; 805 AA.
AC A4SG27;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Cvib_1425;
OS Chlorobium phaeovibrioides (strain DSM 265 / 1930) (Prosthecochloris
OS vibrioformis (strain DSM 265)).
OC Bacteria; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae;
OC Chlorobium/Pelodictyon group; Chlorobium.
OX NCBI_TaxID=290318;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 265 / 1930;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Pitluck S., Schmutz J.,
RA Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J.,
RA Schuster S.C., Bryant D.A., Richardson P.;
RT "Complete sequence of Prosthecochloris vibrioformis DSM 265.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; CP000607; ABP37436.1; -; Genomic_DNA.
DR RefSeq; WP_011890659.1; NC_009337.1.
DR AlphaFoldDB; A4SG27; -.
DR SMR; A4SG27; -.
DR STRING; 290318.Cvib_1425; -.
DR EnsemblBacteria; ABP37436; ABP37436; Cvib_1425.
DR KEGG; pvi:Cvib_1425; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_10; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR Gene3D; 3.90.740.10; -; 1.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..805
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000074839"
FT MOTIF 40..51
FT /note="'HIGH' region"
FT MOTIF 576..580
FT /note="'KMSKS' region"
FT BINDING 579
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 805 AA; 91792 MW; EA7A3A78D650EE00 CRC64;
MRYDFSKTEK KWQARWKQQE TFKTGEAPEK PKYYVLDMFP YPSGSGLHVG HLEGYTASDI
SARYRRSRGY NVLHPMGWDA FGLPAEQFAI KTGTHPSDTT QKNIANFKET LQAMGFSYDW
SREINTTDPG YFQWTQWIFL KLHEMGLAYM SEVDVNWCVE LRAVLANEEV EEKLADGLTV
VRRPLRQWVL KITAYADRLL EDLDGLDWPE NVKQMQRNWI GRSEGVEVDF ELRCHRTMLR
VYTTRPDTLF GATYLVISPE HAMAEKLATA QQLVAVKEYI AKAKLKTELE RTGLQKDKTG
VFTGSYAINP ATGEPLPVWI SDFVLTSYGT GAIMSVPAHD SRDWEFAKQY GLPIIEVVKS
PHDVQQEVFE GKDSTVVNSS NNDISLNGLA FPEAFELMAS WLEKKKAGKR KVNYRLRDWI
FSRQRYWGEP IPIKHYPDGT LKPETSLPLM LPEVEAYQPT ETGESPLANI PEWLNGTDEN
GPFRRETNTM PQWAGSCWYY LRFIDPHNSK LPVDPAKEAY WMNVDLYIGG AEHAVLHLLY
ARFWHKVLFD LKVVSTKEPF QRLFNQGMIL GEDNEKMSKS RGNVIPADHV LQTYGADAVR
LYEMFLGPLE QVKPWNTNGI EGISRFLSRV WRLVWPEPEG TRADLSTLAP SPDLLRRMHK
TIKKVAADTE NLKFNTAIAE MMVFTNELHR TGEGSRIAVE TLLLLLAPYA PHLSEELWEA
LGHQESISLA PFPEFDPELA QDKEVTIAVQ VNGKLRGSFT AAPGSPKEQL LNEAKALEGV
KKFLEGVTIM KEIVVPDKLI NFAVK
