SYL_CHLPN
ID SYL_CHLPN Reviewed; 820 AA.
AC Q9Z930; Q9JQ86;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049};
GN OrderedLocusNames=CPn_0153, CP_0618, CpB0154;
OS Chlamydia pneumoniae (Chlamydophila pneumoniae).
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=83558;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CWL029;
RX PubMed=10192388; DOI=10.1038/7716;
RA Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W.,
RA Olinger L., Grimwood J., Davis R.W., Stephens R.S.;
RT "Comparative genomes of Chlamydia pneumoniae and C. trachomatis.";
RL Nat. Genet. 21:385-389(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AR39;
RX PubMed=10684935; DOI=10.1093/nar/28.6.1397;
RA Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O.,
RA Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S.,
RA Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J.,
RA Gwinn M.L., Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G.,
RA Salzberg S.L., Eisen J.A., Fraser C.M.;
RT "Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae
RT AR39.";
RL Nucleic Acids Res. 28:1397-1406(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J138;
RX PubMed=10871362; DOI=10.1093/nar/28.12.2311;
RA Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K.,
RA Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.;
RT "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from
RT Japan and CWL029 from USA.";
RL Nucleic Acids Res. 28:2311-2314(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TW-183;
RA Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P.,
RA Schneider S., Pohl T., Essig A., Marre R., Melchers K.;
RT "The genome sequence of Chlamydia pneumoniae TW183 and comparison with
RT other Chlamydia strains based on whole genome sequence analysis.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; AE001363; AAD18306.1; -; Genomic_DNA.
DR EMBL; AE002161; AAF38433.1; -; Genomic_DNA.
DR EMBL; BA000008; BAA98363.1; -; Genomic_DNA.
DR EMBL; AE009440; AAP98087.1; -; Genomic_DNA.
DR PIR; A86510; A86510.
DR PIR; C72113; C72113.
DR RefSeq; NP_224361.1; NC_000922.1.
DR RefSeq; WP_010882803.1; NZ_LN847257.1.
DR AlphaFoldDB; Q9Z930; -.
DR SMR; Q9Z930; -.
DR STRING; 115711.CP_0618; -.
DR EnsemblBacteria; AAD18306; AAD18306; CPn_0153.
DR EnsemblBacteria; AAF38433; AAF38433; CP_0618.
DR GeneID; 45050198; -.
DR KEGG; cpa:CP_0618; -.
DR KEGG; cpj:leuS; -.
DR KEGG; cpn:CPn_0153; -.
DR KEGG; cpt:CpB0154; -.
DR PATRIC; fig|115713.3.peg.175; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_0; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000000583; Chromosome.
DR Proteomes; UP000000801; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR Gene3D; 3.90.740.10; -; 1.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..820
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000151998"
FT MOTIF 40..51
FT /note="'HIGH' region"
FT MOTIF 601..605
FT /note="'KMSKS' region"
FT BINDING 604
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 820 AA; 93966 MW; 520369FC098F1926 CRC64;
MRYDPNLIEK KWQQFWKEHR SFQANEDEDK VKYYVLDMFP YPSGAGLHVG HLIGYTATDI
VARYKRARGF SVLHPMGWDS FGLPAEQYAI RTGTHPKVTT QKNIANFKKQ LSAMGFSYDE
GREFATSDPD YYHWTQKLFL FLYDQGLAYM ADMAVNYCPE LGTVLSNEEV ENGFSIEGGY
PVERKMLRQW ILKITAYADK LLEGLDALDW PENVKQLQKN WIGKSEGALV TFHLTQEGSL
EAFTTRLDTL LGVSFLVIAP EHPDLDSIVS EEQRDEVTAY VQESLRKSER DRISSVKTKT
GVFTGNYAKH PITGNLLPVW ISDYVVLGYG TGVVMGVPAH DERDREFAEM FSLPIHEVID
DNGVCIHSNY NDFCLNGLSG QEAKDYVINY LEMRSLGRAK TMYRLRDWLF SRQRYWGEPI
PIIHFEDGTH RPLEDDELPL LPPNIDDYRP EGFGQGPLAK AQDWVHIYDE KTGRPGCRET
YTMPQWAGSC WYYLRFCDAH NSQLPWSKEK ESYWMPVDLY IGGAEHAVLH LLYSRFWHRV
FYDAGLVSTP EPFKKLINQG LVLASSYRIP GKGYVSIEDV REENGTWIST CGEIVEVRQE
KMSKSKLNGV DPQVLIEEYG ADALRMYAMF SGPLDKNKTW SNEGVGGCRR FLNRFYDLVT
SSEVQDIEDR DGLVLAHKLV FRITEHIEKM SLNTIPSSFM EFLNDFSKLP VYSKRALSMA
VRVLEPIAPH ISEELWVILG NPPGIDQAAW PQIDESYLVA QTVTFVVQVN GKLRGRLEVA
KEAPKEEVLS LSRSVVAKYL ENAQIRKEIY VPNKLVNFVL
