ID   SYL_CHRSD               Reviewed;         856 AA.
AC   Q1QV15;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Csal_2344;
OS   Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 /
OS   NCIMB 13768 / 1H11).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales;
OC   Halomonadaceae; Chromohalobacter.
OX   NCBI_TaxID=290398;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11;
RX   PubMed=22675587; DOI=10.4056/sigs.2285059;
RA   Copeland A., O'Connor K., Lucas S., Lapidus A., Berry K.W., Detter J.C.,
RA   Del Rio T.G., Hammon N., Dalin E., Tice H., Pitluck S., Bruce D.,
RA   Goodwin L., Han C., Tapia R., Saunders E., Schmutz J., Brettin T.,
RA   Larimer F., Land M., Hauser L., Vargas C., Nieto J.J., Kyrpides N.C.,
RA   Ivanova N., Goker M., Klenk H.P., Csonka L.N., Woyke T.;
RT   "Complete genome sequence of the halophilic and highly halotolerant
RT   Chromohalobacter salexigens type strain (1H11(T)).";
RL   Stand. Genomic Sci. 5:379-388(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000285; ABE59693.1; -; Genomic_DNA.
DR   RefSeq; WP_011507639.1; NC_007963.1.
DR   AlphaFoldDB; Q1QV15; -.
DR   SMR; Q1QV15; -.
DR   STRING; 290398.Csal_2344; -.
DR   PRIDE; Q1QV15; -.
DR   EnsemblBacteria; ABE59693; ABE59693; Csal_2344.
DR   KEGG; csa:Csal_2344; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_6; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000000239; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 3.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..856
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009324"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           615..619
FT                   /note="'KMSKS' region"
FT   BINDING         618
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   856 AA;  95491 MW;  37952B02BD302926 CRC64;
     MDAQYTPHAI ESAAQTFWDK NQCFKAVEDA TREKFYCLSM FPYPSGKLHM GHVRNYTIGD
     VVSRFQRMQG KNVLQPMGWD AFGMPAENAA IKNRVPPGAW TYDNIDAMRR QLKALGFAYD
     WNREFATCDV DYYRWEQWFF TKLVEKGLVY KKMSTVNWDP VDQTVLANEQ VIEGRGWRSG
     ALVERKEIPL WFLKITDYAD ELLADLDKVD WPEQVKTMQR NWIGKSTGVE LSFAVADSSE
     QLEVYTTRPD TLYGVTYVAV AAGHPLARQA AQDNPALGDF LEECQQGGNS EAELATMEKK
     GMDTGHKAIH PLTGREVPIF VANFVLMEYG TGAVMAVPAH DQRDWEFATK YGIPIEPVIA
     DAEGNTPDLS QGAHTEHGKL INSGEFDGLE FDAAFDAIAA RLEANGQGTV KTNFRLRDWG
     VARQRYWGAP IPVKYGPEGQ TVPLTDDELP VALPMEVEVD ASGSPLKKMP AFYDLGEGWT
     RETDTFDTFM ESSWYYARFA SADNMEAMLD ERADYWLPVD LYIGGIEHAI LHLLYARFFH
     KLMRDFGLVA SDEPFQRLLT QGMVIAETFY RANDDGSKDW FNPADVDVQR DDKGRPVSAI
     LREDGQPVEM GGIEKMSKSK NNGVDPQAMI DRFGADTVRL FMMFAAPPEQ SLEWSDSGVE
     GAHRFLKRLW KLVADHLDAG TPAALDADAL NDDQKTLRRK THETIAKASD DIGRRTTFNT
     AIAAVMELVN AIGRFEDTSP QGLAVTREAL EACVLVLAPI VPHACHALWD ALGHDTPVID
     AAWPQADEAA MVKDSVELAV QVNGKLRARL DVPAAADKAA IEAQALEAEN VRRHTEGKTI
     RKVIVVPGKL VNIVAN