ID   SYL_CITBB               Reviewed;         824 AA.
AC   B5EE39;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Gbem_0751;
OS   Citrifermentans bemidjiense (strain ATCC BAA-1014 / DSM 16622 / JCM 12645 /
OS   Bem) (Geobacter bemidjiensis).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC   Geobacteraceae; Citrifermentans.
OX   NCBI_TaxID=404380;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1014 / DSM 16622 / JCM 12645 / Bem;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Lykidis A., Lovley D., Richardson P.;
RT   "Complete sequence of Geobacter bemidjiensis BEM.";
RL   Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP001124; ACH37777.1; -; Genomic_DNA.
DR   RefSeq; WP_012529184.1; NC_011146.1.
DR   AlphaFoldDB; B5EE39; -.
DR   SMR; B5EE39; -.
DR   STRING; 404380.Gbem_0751; -.
DR   EnsemblBacteria; ACH37777; ACH37777; Gbem_0751.
DR   KEGG; gbm:Gbem_0751; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_7; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000008825; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 2.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 2.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..824
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000091321"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           581..585
FT                   /note="'KMSKS' region"
FT   BINDING         584
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   824 AA;  92622 MW;  8D2FC08F5B069D1C CRC64;
     MEEKYVPSAV EEKWQGYWAE HKSFKANEDP SRKKYYLLEM FPYPSGKIHM GHVRNYSIGD
     VIARFKRMQG YNVLHPMGWD AFGMPAENAA IQHKSHPAKW TYENIAYMRG QLKTLGLSYD
     WDRELATCDL DYYKWEQRIF LEMYKKGLAY KKSSAVNWCP KCETVLANEQ VEDGCCWRCD
     SPVQQKELEQ WSFRITNYAQ ELLDDTYKLT GWPERVLTMQ RNWIGRSTGC EIDFPLESGL
     GKIKVFTTRQ DTLFGATFMS LAAEHPMALD LAGDAQKAEV AAFIDKVKKT DRIKRGAEDL
     EKEGVFTGSY CINPVTNTKM PIYLANFVLM DYGTGAVMAV PTHDQRDFEF AKKYNLPLKV
     VIQPEGETLD PATMTEAYTA EGIMVNSGRF DGMGNADAKE AIADFLEKEG IGKKTVNFRL
     RDWGISRQRY WGNPIPVINC DLCGVVAVPE ADLPVVLPMD AEFTGEGGNP LARVDSFTTC
     TCPQCGETAR RETDTMDTFV QSSWYFLRYC SPKFSAGPLD REKVEAWMPV DQYIGGIEHA
     VLHLLYARFF TKVLRDLGYC NVDEPFSNLL TQGMVIKDGA KMSKSKGNVV DPNALIERYG
     ADTARLFSLF AAPPEKDLDW SDQGVDGSYR FLNRVWRLVY DVLPVIGDAG AVNPGELGAE
     AKKLRRAVHK TIKKVSEDVE ERFHFNTAIA AVMELVNAIQ AFSAKDAPEN VAVVREAVES
     VVRLLAPFVP NFAEELWFQL GHASVLEAAG WPGYDAAAVV DEEVTVVIQV NGKLRSKLTV
     APDAKEEEVR AQALADDKIK PYLEGKDVKK VVYVPGKLVS IVVA