ID   SYL_COLP3               Reviewed;         863 AA.
AC   Q484Q5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=CPS_1722;
OS   Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio
OS   psychroerythus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Colwelliaceae; Colwellia.
OX   NCBI_TaxID=167879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=34H / ATCC BAA-681;
RX   PubMed=16043709; DOI=10.1073/pnas.0504766102;
RA   Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X.,
RA   Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M.,
RA   Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A.,
RA   Zhou L., Davidsen T.M., Wu M., Huston A.L., Lewis M., Weaver B.,
RA   Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.;
RT   "The psychrophilic lifestyle as revealed by the genome sequence of
RT   Colwellia psychrerythraea 34H through genomic and proteomic analyses.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000083; AAZ26868.1; -; Genomic_DNA.
DR   RefSeq; WP_011042554.1; NC_003910.7.
DR   AlphaFoldDB; Q484Q5; -.
DR   SMR; Q484Q5; -.
DR   STRING; 167879.CPS_1722; -.
DR   PRIDE; Q484Q5; -.
DR   EnsemblBacteria; AAZ26868; AAZ26868; CPS_1722.
DR   KEGG; cps:CPS_1722; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_6; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000000547; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 3.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..863
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009332"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           618..622
FT                   /note="'KMSKS' region"
FT   BINDING         621
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   863 AA;  97091 MW;  86F7EE2A5585161F CRC64;
     MEAIYNPQAI EATVQKFWTD NNTFQAIENP DKEKFYCLAM FPYPSGRLHM GHVRNYSLGD
     VISRYQRMQG KNVMQPMGWD AFGLPAENAA IKNKTAPGKW TYENIDYMRN QLQSLGFGYD
     WGRELATCKP DYYRWEQWFF TQLFEKGLVY KKNATVNWDP VDQTVLANEQ VIDGRGWRSG
     ALVERKQIPQ WFIKITDYAE ELLDDLDQLT EWPEQVKTMQ RNWIGRSQGV EMTFAVADST
     ESFDIYTTRP DTLMGVTYVA LAAQHPLAVA AAVDNADLAA FIDECKNSKT TEADMAAMEK
     KGVDTGLKAI HPLTGKLVPV WAANFVLMDY GSGAVMSVPG HDQRDYEFAL KYGLAIEQVI
     AGQEADDINK AAITEKSTLI NSGEFDGLDF EEAFKAISDK LISENKGKTT TNYRLRDWGV
     SRQRYWGTPI PMINLANGES VPVPTNELPV VLPEDVVMNG TTSPIKADPE WAKTLYNGEE
     ALRETDTFDT FMESSWYYAR YCSPNDDTQM IDPAKANYWL PVDQYIGGIE HAILHLLYSR
     FFHKLLRDVG LVKCDEPFKK LLCQGMVLAE TYYREADNGA QEWIAPTDVE VERDEKGQIT
     SSISKIDGQP VLSAGMSKMS KSKNNGIDPQ EVIEKYGADT VRLFIMFTSP PEQTLEWSDA
     GVEGAHRFVK RVYKLAHEFV ESTNNSAVVD IAELTLNADH KKLRRELHKT IAKVTDDIGR
     RNTFNTAIAA IMELMNHLGK AKVNSDEDKA VMQEAVRAVV LMLTPITPHL CHHLWQLVGG
     SDENVEDASW PVVDNSALVE DEKLIIVQVN GKVRAKITVA ADASKEDVEA LGLNDESVLK
     FTDGNTIRKV IYIPGKLLNI VAN