SYL_CORA7
ID SYL_CORA7 Reviewed; 949 AA.
AC C3PKU4;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=cauri_2469;
OS Corynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CIP 107346 /
OS CN-1) (Corynebacterium nigricans).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium.
OX NCBI_TaxID=548476;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700975 / DSM 44827 / CIP 107346 / CN-1;
RX PubMed=20137072; DOI=10.1186/1471-2164-11-91;
RA Trost E., Gotker S., Schneider J., Schneiker-Bekel S., Szczepanowski R.,
RA Tilker A., Viehoever P., Arnold W., Bekel T., Blom J., Gartemann K.H.,
RA Linke B., Goesmann A., Puhler A., Shukla S.K., Tauch A.;
RT "Complete genome sequence and lifestyle of black-pigmented Corynebacterium
RT aurimucosum ATCC 700975 (formerly C. nigricans CN-1) isolated from a
RT vaginal swab of a woman with spontaneous abortion.";
RL BMC Genomics 11:91-91(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; CP001601; ACP34060.1; -; Genomic_DNA.
DR RefSeq; WP_010187942.1; NZ_ACLH01000027.1.
DR AlphaFoldDB; C3PKU4; -.
DR SMR; C3PKU4; -.
DR STRING; 548476.cauri_2469; -.
DR PRIDE; C3PKU4; -.
DR EnsemblBacteria; ACP34060; ACP34060; cauri_2469.
DR GeneID; 31925117; -.
DR KEGG; car:cauri_2469; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_11; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000002077; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 3.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..949
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000199188"
FT REGION 542..561
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 62..73
FT /note="'HIGH' region"
FT MOTIF 719..723
FT /note="'KMSKS' region"
FT BINDING 722
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 949 AA; 107536 MW; 740B6BD315B1ECE1 CRC64;
MTNPSDNTTH RYTPELAAQI ESAWQQYWKD NGTFNAPNPV GPLAEEGKEL PAEKMNIQDM
FPYPSGAGLH VGHPLGYIAT DTYARFNRML GKNVLHTLGY DAFGLPAEQY AIQTGTHPRT
TTEANINNMR RQLGMLGLGH DPRRSVASTD PEFYKWTQWI FLQIYNSWFD EEQQKARPIS
ELIKELEVGK RTTKDGRYYA DLTKEEQRQA LDEFRLVYLS NSTVNWCPGL GTVLANEEVT
AEGKSERGNF PVFRKNLRQW MMRITAYSDR LLDDLELLDW PEKVKSMQRN WIGRSRGAEV
SFHAEGYNID VFTTRPDTLF GAEYVVLAPE HELVDALLSP IPYEDDVDKR WTFGHDDPKE
AVEAYRASIA AKSDLERQEN KEKTGVFLGT YATNPVNGKQ IPIFIADYVL TGYGTGAIMA
VPAHDTRDYE FATVFGLPIT EVVAGGDITK EAYTESGQAV NSANDSLDIN GMSKDEAIAT
IIPWLEEQGV GREKIQYKLR DWLFARQRYW GEPFPIVYDE AGQAYPLPES MLPIELPEVE
DYKPVSFDPD DADSSPQPPL AKAREWVEVE LDLGLGDGPK TYYRDTNVMP QWAGSSWYQL
RYIDPTNDEI FCDLENERYW TGPRTEEHGA NDPGGVDLYV GGVEHAVLHL LYARFWHKVL
FDLGYVSSKE PYRRLYNQGY IQAYAYTDSR GVYVPAAEVE EKDGKFYYNG EEVNQEYGKM
GKSLKNSVAP DDICRDYGAD TLRVYEMSMG PLDTSRPWAT KDVVGAQRFL QRLWRLAIDE
NSGELSTTDA ELSEDDLKHL NRTIAGVRDD YENLRLNTVV AKLIEYVNYL TKAYPKGAPR
AAVEPLAQLV SPVAPHIAEE LWKRFGHEGT ITYEAFPEFD EKYLVDDEIE VPVQINGKVK
ARVMVPADAD QATVVGIAKD DERIAELTAG KNVVKEIYVP GRMVNLVVK
