SYL_DESAP
ID SYL_DESAP Reviewed; 826 AA.
AC B1I5R7;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Daud_1865;
OS Desulforudis audaxviator (strain MP104C).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae;
OC Candidatus Desulforudis.
OX NCBI_TaxID=477974;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MP104C;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Bruce D., Pitluck S., Lowry S.R., Larimer F., Land M.L.,
RA Hauser L., Kyrpides N., Ivanova N.N., Richardson P.;
RT "Complete sequence of chromosome of Desulforudis audaxviator MP104C.";
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; CP000860; ACA60358.1; -; Genomic_DNA.
DR RefSeq; WP_012302934.1; NC_010424.1.
DR AlphaFoldDB; B1I5R7; -.
DR SMR; B1I5R7; -.
DR STRING; 477974.Daud_1865; -.
DR PRIDE; B1I5R7; -.
DR EnsemblBacteria; ACA60358; ACA60358; Daud_1865.
DR KEGG; dau:Daud_1865; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_9; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000008544; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 2.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..826
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000091313"
FT MOTIF 42..52
FT /note="'HIGH' region"
FT MOTIF 581..585
FT /note="'KMSKS' region"
FT BINDING 584
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 826 AA; 93865 MW; 6363D3EDDC6C95AA CRC64;
MEEKYVFAEI EEKLRRRWET EGIYHVPDFS NRPKYYCLEM FPYPSGNLHM GHVRNYAIGD
VVARFKTMRG YDVLHPMGWD AFGLPAENAA IQHGVPPARW TWDNINVMRT QLKLLGVSYD
WRRELATCHP GYYRWTQWLF LQFYHRGLAY KAKAPVNWCP SCATVLANEQ VVAGGCERCK
TAVERRELEQ WFFRITAYAD RLLKDLAKLP GWPEKVKVMQ ENWIGRSTGA EIAFPLAGTD
EAIRVFTTRP DTLGGVTYMT IAPEHPLVSR VTTPEHRAAV EAFTERARSL SELDRTAGEH
EKEGLFTGAY CVNPLTGEQV PVFVANYVLM EYGTGCVMGV PAHDQRDFEF ARKYGLPVRV
VIQPEGDLLD GDTMSQAYTG PGRLVNTPGF DRMANAEAIP AITRYLEARG AARFQVQYRL
RDWLISRQRY WGAPIPIVYC EGCGVVPVPE EGLPVLLPED VAFKPTGRSP LTESPDFVNT
TCPTCGGPAR RETDTMDTFM CSSWYYFRFT SPREENGPWG LERVDRWLPV DQYIGGVEHA
ILHLMYSRFF TKVLYDMGLV KVQEPFTNLL TQGMVLKDGA KMSKSKGNVV SPEDILNRYG
ADTTRLFVLF AAPPERDLEW SDQGVEGCYR FLQRVWRLVN SVADEIRGAA PVPSANLVGV
NRSMRRLTHQ TIKKVTEDIE TRFNFNTAIS AAMELVNGMY HFRDRVAPVN RDPAVMREAV
ERLLLLLAPF APFLADELWA RTGHPESIHR EPWPEYDPEL LVEDQVEIVV QINGRVRDRL
MVAADIAPEA MRDTVLEQPR VQALVAGKEI VKVVPVPGKL VNIVVR
