ID   SYL_DICNV               Reviewed;         867 AA.
AC   A5EWD6;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=DNO_0246;
OS   Dichelobacter nodosus (strain VCS1703A).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Cardiobacteriales;
OC   Cardiobacteriaceae; Dichelobacter.
OX   NCBI_TaxID=246195;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VCS1703A;
RX   PubMed=17468768; DOI=10.1038/nbt1302;
RA   Myers G.S.A., Parker D., Al-Hasani K., Kennan R.M., Seemann T., Ren Q.,
RA   Badger J.H., Selengut J.D., Deboy R.T., Tettelin H., Boyce J.D.,
RA   McCarl V.P., Han X., Nelson W.C., Madupu R., Mohamoud Y., Holley T.,
RA   Fedorova N., Khouri H., Bottomley S.P., Whittington R.J., Adler B.,
RA   Songer J.G., Rood J.I., Paulsen I.T.;
RT   "Genome sequence and identification of candidate vaccine antigens from the
RT   animal pathogen Dichelobacter nodosus.";
RL   Nat. Biotechnol. 25:569-575(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000513; ABQ13397.1; -; Genomic_DNA.
DR   RefSeq; WP_012030594.1; NC_009446.1.
DR   AlphaFoldDB; A5EWD6; -.
DR   SMR; A5EWD6; -.
DR   STRING; 246195.DNO_0246; -.
DR   EnsemblBacteria; ABQ13397; ABQ13397; DNO_0246.
DR   KEGG; dno:DNO_0246; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_6; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000000248; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 2.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..867
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009336"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           631..635
FT                   /note="'KMSKS' region"
FT   BINDING         634
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   867 AA;  99087 MW;  E394F561913ABF7D CRC64;
     MQDTYQPAQI EAAIQQQWAN EERFKAVVNP QKEKFYCLSM FPYPSGKLHM GHVRNYTIGD
     VISRYQRMLG KNVLQPMGWD AFGMPAENAA MKNQVAPAQW TYQNIAEMKK QLQSLGFAID
     WSREITTCRP DYYRWEQWLF TRLYQKGVIY RKLGAVNWDP VDQTVLANEQ VIDGRGWRSG
     AIVEKREIPM YYFRITDYAE ELLNDLEQLD GWPERVKMMQ RNWIGKSRGM TIRFPIEPES
     QKGLSAENAQ FLQIYTTRPD TIFGVTFLAV AAEHPLALAA AEDNPALRQF MTECKTGSVA
     EADLATMPKK GMATGRFVTH PLTGKRLPVW VGNYVLSGYG DAAVMGVPAH DERDFHFAHQ
     YGLPIVQVIQ TEKDLPAFNA DSWQEWYGDK EEMHVIASDD CNGLNYDQAF EKLSEKLAAL
     NLGEPKTQYR LRDWGISRQR YWGCPIPIIH HDDGEALADT LPVVLPEDKI PDGGGSVLAQ
     SPDYYECRFQ GKAARRETDT MDTFVESSWY QFRYMSPHYQ KGMLDPEEVA YWQDVDQYIG
     GIEHAILHLL YARFFTKLLR DEGLVHTDEP FKKLLTQGMV LAGTWYREQE HGSKIWFNPA
     DVQVKTDDKG RIIGGVLLSD GKPVQYGGME KMSKSKNNGV DPQALIKTYG ADTARLYTMF
     TAPPEASLEW SESGVEGAYR FLRRLWAYCY EFRHSIAKPS EVVPLNEKHQ NIRREIHEQL
     RAARFDYERN QFNTVVSAGM KLFNCLNEIE PDYDALRREG VRILLLILSP IVPHITETLW
     QALDFSGVIS DQALPEVDET ALKQDNMTLV VQINGKRRGE ISVATDAAQQ NIIDCALADE
     RFAPYLAGLT VQKTIYVPKK LVNIVAK