SYL_ECOLI
ID SYL_ECOLI Reviewed; 860 AA.
AC P07813; P77110; P78292;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 195.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049};
GN OrderedLocusNames=b0642, JW0637;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3320963; DOI=10.1093/nar/15.24.10199;
RA Haertlein M., Madern D.;
RT "Molecular cloning and nucleotide sequence of the gene for Escherichia coli
RT leucyl-tRNA synthetase.";
RL Nucleic Acids Res. 15:10199-10210(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBUNIT: Monomer.
CC -!- INTERACTION:
CC P07813; P33129: htrE; NbExp=3; IntAct=EBI-553345, EBI-550887;
CC P07813; P15042: ligA; NbExp=3; IntAct=EBI-553345, EBI-553496;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB40843.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X06331; CAA29642.1; -; Genomic_DNA.
DR EMBL; U82598; AAB40843.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC73743.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35289.1; -; Genomic_DNA.
DR PIR; H64798; SYECL.
DR RefSeq; NP_415175.1; NC_000913.3.
DR RefSeq; WP_001340834.1; NZ_SSZK01000037.1.
DR PDB; 2AJG; X-ray; 2.00 A; A/B=228-413.
DR PDB; 2AJH; X-ray; 2.40 A; A/B=228-413.
DR PDB; 2AJI; X-ray; 3.20 A; A/B=228-413.
DR PDB; 3ZGZ; X-ray; 2.40 A; A/D=1-860.
DR PDB; 3ZJT; X-ray; 2.20 A; A=1-860.
DR PDB; 3ZJU; X-ray; 2.40 A; A=1-860.
DR PDB; 3ZJV; X-ray; 2.31 A; A=1-860.
DR PDB; 4AQ7; X-ray; 2.50 A; A/D=1-860.
DR PDB; 4ARC; X-ray; 2.00 A; A=1-860.
DR PDB; 4ARI; X-ray; 2.08 A; A=1-860.
DR PDB; 4AS1; X-ray; 2.02 A; A=1-860.
DR PDB; 4CQN; X-ray; 2.50 A; A/D=1-860.
DR PDB; 5OMW; X-ray; 2.60 A; A/D=1-860.
DR PDB; 5ON2; X-ray; 3.10 A; A/D=1-860.
DR PDB; 5ON3; X-ray; 3.10 A; A/D=1-860.
DR PDB; 5ONH; X-ray; 3.10 A; A/D=1-860.
DR PDBsum; 2AJG; -.
DR PDBsum; 2AJH; -.
DR PDBsum; 2AJI; -.
DR PDBsum; 3ZGZ; -.
DR PDBsum; 3ZJT; -.
DR PDBsum; 3ZJU; -.
DR PDBsum; 3ZJV; -.
DR PDBsum; 4AQ7; -.
DR PDBsum; 4ARC; -.
DR PDBsum; 4ARI; -.
DR PDBsum; 4AS1; -.
DR PDBsum; 4CQN; -.
DR PDBsum; 5OMW; -.
DR PDBsum; 5ON2; -.
DR PDBsum; 5ON3; -.
DR PDBsum; 5ONH; -.
DR AlphaFoldDB; P07813; -.
DR SMR; P07813; -.
DR BioGRID; 4263356; 59.
DR BioGRID; 851814; 7.
DR DIP; DIP-10095N; -.
DR IntAct; P07813; 21.
DR STRING; 511145.b0642; -.
DR BindingDB; P07813; -.
DR ChEMBL; CHEMBL4295566; -.
DR ChEMBL; CHEMBL4662935; -.
DR CarbonylDB; P07813; -.
DR SWISS-2DPAGE; P07813; -.
DR jPOST; P07813; -.
DR PaxDb; P07813; -.
DR PRIDE; P07813; -.
DR EnsemblBacteria; AAC73743; AAC73743; b0642.
DR EnsemblBacteria; BAA35289; BAA35289; BAA35289.
DR GeneID; 947497; -.
DR KEGG; ecj:JW0637; -.
DR KEGG; eco:b0642; -.
DR PATRIC; fig|1411691.4.peg.1626; -.
DR EchoBASE; EB0527; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_6; -.
DR InParanoid; P07813; -.
DR OMA; TFMVLAP; -.
DR PhylomeDB; P07813; -.
DR BioCyc; EcoCyc:LEUS-MON; -.
DR BioCyc; MetaCyc:LEUS-MON; -.
DR BRENDA; 6.1.1.4; 2026.
DR SABIO-RK; P07813; -.
DR EvolutionaryTrace; P07813; -.
DR PRO; PR:P07813; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IDA:EcoCyc.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IDA:EcoCyc.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 3.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..860
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000152012"
FT MOTIF 42..52
FT /note="'HIGH' region"
FT MOTIF 619..623
FT /note="'KMSKS' region"
FT BINDING 622
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
FT CONFLICT 67
FT /note="R -> H (in Ref. 1; CAA29642)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="T -> N (in Ref. 1; CAA29642)"
FT /evidence="ECO:0000305"
FT CONFLICT 262
FT /note="A -> R (in Ref. 1 and 3)"
FT /evidence="ECO:0000305"
FT HELIX 7..20
FT /evidence="ECO:0007829|PDB:4ARC"
FT TURN 21..24
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:4CQN"
FT STRAND 34..39
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 45..47
FT /evidence="ECO:0007829|PDB:3ZJT"
FT HELIX 50..68
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 76..79
FT /evidence="ECO:0007829|PDB:4AQ7"
FT HELIX 85..92
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 93..95
FT /evidence="ECO:0007829|PDB:3ZJU"
FT HELIX 97..114
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 121..123
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 130..145
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 149..154
FT /evidence="ECO:0007829|PDB:4ARC"
FT TURN 160..163
FT /evidence="ECO:0007829|PDB:3ZGZ"
FT STRAND 164..166
FT /evidence="ECO:0007829|PDB:3ZGZ"
FT HELIX 168..170
FT /evidence="ECO:0007829|PDB:3ZGZ"
FT STRAND 177..179
FT /evidence="ECO:0007829|PDB:3ZGZ"
FT STRAND 188..193
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 195..198
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 199..205
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 206..208
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 214..224
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 229..236
FT /evidence="ECO:0007829|PDB:2AJG"
FT STRAND 240..248
FT /evidence="ECO:0007829|PDB:2AJG"
FT HELIX 250..255
FT /evidence="ECO:0007829|PDB:2AJG"
FT STRAND 258..261
FT /evidence="ECO:0007829|PDB:2AJG"
FT HELIX 266..272
FT /evidence="ECO:0007829|PDB:2AJG"
FT HELIX 276..287
FT /evidence="ECO:0007829|PDB:2AJG"
FT HELIX 292..297
FT /evidence="ECO:0007829|PDB:2AJG"
FT STRAND 302..310
FT /evidence="ECO:0007829|PDB:2AJG"
FT TURN 312..314
FT /evidence="ECO:0007829|PDB:2AJG"
FT STRAND 317..323
FT /evidence="ECO:0007829|PDB:2AJG"
FT STRAND 328..331
FT /evidence="ECO:0007829|PDB:2AJG"
FT STRAND 333..337
FT /evidence="ECO:0007829|PDB:2AJG"
FT TURN 339..341
FT /evidence="ECO:0007829|PDB:2AJG"
FT HELIX 343..352
FT /evidence="ECO:0007829|PDB:2AJG"
FT STRAND 371..373
FT /evidence="ECO:0007829|PDB:2AJG"
FT HELIX 385..387
FT /evidence="ECO:0007829|PDB:2AJG"
FT HELIX 392..405
FT /evidence="ECO:0007829|PDB:2AJG"
FT STRAND 408..412
FT /evidence="ECO:0007829|PDB:2AJG"
FT STRAND 423..425
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 427..429
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 434..437
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 442..444
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 447..449
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 462..464
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 466..469
FT /evidence="ECO:0007829|PDB:4ARC"
FT TURN 471..474
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 475..485
FT /evidence="ECO:0007829|PDB:4ARC"
FT TURN 492..494
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 495..497
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 499..502
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 510..512
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 515..521
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 523..528
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 531..533
FT /evidence="ECO:0007829|PDB:4ARC"
FT TURN 534..536
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 537..550
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 559..564
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 569..577
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 579..581
FT /evidence="ECO:0007829|PDB:4CQN"
FT STRAND 583..586
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 588..590
FT /evidence="ECO:0007829|PDB:4AS1"
FT STRAND 592..595
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 597..599
FT /evidence="ECO:0007829|PDB:4AQ7"
FT STRAND 601..605
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 613..619
FT /evidence="ECO:0007829|PDB:4ARC"
FT TURN 622..625
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 630..637
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 639..648
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 660..682
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 691..693
FT /evidence="ECO:0007829|PDB:4AS1"
FT HELIX 696..717
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 722..737
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 744..761
FT /evidence="ECO:0007829|PDB:4ARC"
FT TURN 762..764
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 766..775
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 782..784
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 792..795
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 798..806
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 809..817
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 822..831
FT /evidence="ECO:0007829|PDB:4ARC"
FT HELIX 833..836
FT /evidence="ECO:0007829|PDB:4ARC"
FT TURN 837..841
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 846..850
FT /evidence="ECO:0007829|PDB:4ARC"
FT TURN 851..853
FT /evidence="ECO:0007829|PDB:4ARC"
FT STRAND 854..858
FT /evidence="ECO:0007829|PDB:4ARC"
SQ SEQUENCE 860 AA; 97234 MW; 856BBEDECC03C83C CRC64;
MQEQYRPEEI ESKVQLHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM GHVRNYTIGD
VIARYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYDNIAYMKN QLKMLGFGYD
WSRELATCTP EYYRWEQKFF TELYKKGLVY KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD
TKVERKEIPQ WFIKITAYAD ELLNDLDKLD HWPDTVKTMQ RNWIGRSEGV EITFNVNDYD
NTLTVYTTRP DTFMGCTYLA VAAGHPLAQK AAENNPELAA FIDECRNTKV AEAEMATMEK
KGVDTGFKAV HPLTGEEIPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAS KYGLNIKPVI
LAADGSEPDL SQQALTEKGV LFNSGEFNGL DHEAAFNAIA DKLTAMGVGE RKVNYRLRDW
GVSRQRYWGA PIPMVTLEDG TVMPTPDDQL PVILPEDVVM DGITSPIKAD PEWAKTTVNG
MPALRETDTF DTFMESSWYY ARYTCPQYKE GMLDSEAANY WLPVDIYIGG IEHAIMHLLY
FRFFHKLMRD AGMVNSDEPA KQLLCQGMVL ADAFYYVGEN GERNWVSPVD AIVERDEKGR
IVKAKDAAGH ELVYTGMSKM SKSKNNGIDP QVMVERYGAD TVRLFMMFAS PADMTLEWQE
SGVEGANRFL KRVWKLVYEH TAKGDVAALN VDALTENQKA LRRDVHKTIA KVTDDIGRRQ
TFNTAIAAIM ELMNKLAKAP TDGEQDRALM QEALLAVVRM LNPFTPHICF TLWQELKGEG
DIDNAPWPVA DEKAMVEDST LVVVQVNGKV RAKITVPVDA TEEQVRERAG QEHLVAKYLD
GVTVRKVIYV PGKLLNLVVG
