SYL_ENTFA
ID SYL_ENTFA Reviewed; 804 AA.
AC Q837N5;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=EF_0801;
OS Enterococcus faecalis (strain ATCC 700802 / V583).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=226185;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700802 / V583;
RX PubMed=12663927; DOI=10.1126/science.1080613;
RA Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT faecalis.";
RL Science 299:2071-2074(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; AE016830; AAO80614.1; -; Genomic_DNA.
DR RefSeq; NP_814544.1; NC_004668.1.
DR RefSeq; WP_010706700.1; NZ_KE136527.1.
DR AlphaFoldDB; Q837N5; -.
DR SMR; Q837N5; -.
DR STRING; 226185.EF_0801; -.
DR EnsemblBacteria; AAO80614; AAO80614; EF_0801.
DR KEGG; efa:EF0801; -.
DR PATRIC; fig|226185.45.peg.2737; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_9; -.
DR OMA; TFMVLAP; -.
DR Proteomes; UP000001415; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR Gene3D; 3.90.740.10; -; 1.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..804
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000152015"
FT MOTIF 40..51
FT /note="'HIGH' region"
FT MOTIF 576..580
FT /note="'KMSKS' region"
FT BINDING 579
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 804 AA; 91839 MW; C79970F0DDFC14B5 CRC64;
MSYNHKEIEK KWQKYWAKNN CFNTLDDPNK EKFYALDMFP YPSGQGLHVG HPEGYTATDI
LSRMKRAQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAEFT KKNIETFRRQ INSLGFSYDW
NREINTTDPE YYKWTQWIFT KLYEKGLAYE AEVAVNWVPE LGTVISNEEV IDGKSERGGY
DVVRRPMRQW MLKITAYADR LLEDLELVDW PESIKDMQRN WIGRSEGANV TFKVAGTEES
FTVFTTRPDT LFGATYTVLA PELELVKKIT TPEQTAAVEA YIEETSKKSD LNRTDLAKEK
TGVFTGAYAI NPVNGQEIPI WIGDYVLASY GTGAIMAVPA HDERDYEFAK TFGIDILPVI
AGGDITTEAY TGDGPHINSD FLNGLNKAEA IAKMNEWLEE NHVGKKEVSY RLRDWLFSRQ
RYWGEPIPVI HWEDGTTTTV PESELPLRLP VTSDIRPSGT GESPLANIDE WVNVVDPETG
MKGKRETNTM PQWAGSSWYY LRFIDPHNKN EIADFEKLKR WLPVDIYIGG AEHAVLHLLY
ARFWHKFLYD IGVVPTKEPF QKLYNQGMIL GENNEKMSKS RGNVVNPDDV VAKYGADTLR
LYEMFMGPLD ASIAWNENGL EGSRKFLDRV WRLIVDEEGK MRDRITTIND GRLTKVYHQT
VKKVTEDMAN LHFNTAISQL MVFVNEANKV DALPYEYVEG FVQLLAPIAP HIGEELWQIL
GNEESLTYVP WPTYDETALV EDEVEVVFQV NGKLRGKQNV ARGLSKEELE QIAMNHEAVK
EFIEGKTVRK VIAVPDKLVN IVAN
