ID   SYL_GEOKA               Reviewed;         805 AA.
AC   Q5KW09;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=GK2842;
OS   Geobacillus kaustophilus (strain HTA426).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC   Geobacillus thermoleovorans group.
OX   NCBI_TaxID=235909;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HTA426;
RX   PubMed=15576355; DOI=10.1093/nar/gkh970;
RA   Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H.,
RA   Matsui S., Uchiyama I.;
RT   "Thermoadaptation trait revealed by the genome sequence of thermophilic
RT   Geobacillus kaustophilus.";
RL   Nucleic Acids Res. 32:6292-6303(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; BA000043; BAD77127.1; -; Genomic_DNA.
DR   RefSeq; WP_011232314.1; NC_006510.1.
DR   AlphaFoldDB; Q5KW09; -.
DR   SMR; Q5KW09; -.
DR   STRING; 235909.GK2842; -.
DR   PRIDE; Q5KW09; -.
DR   EnsemblBacteria; BAD77127; BAD77127; GK2842.
DR   KEGG; gka:GK2842; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_9; -.
DR   OMA; TFMVLAP; -.
DR   Proteomes; UP000001172; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..805
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000152019"
FT   MOTIF           40..51
FT                   /note="'HIGH' region"
FT   MOTIF           576..580
FT                   /note="'KMSKS' region"
FT   BINDING         579
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   805 AA;  92875 MW;  C5D51D58D9C78270 CRC64;
     MSFNHREIEQ KWQDYWEKNK TFRTPDDDDK PKFYVLDMFP YPSGAGLHVG HPEGYTATDI
     LARMKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAEFT QKNIDNFRRQ IKSLGFSYDW
     DREINTTDPN YYKWTQWIFL KLYEKGLAYM DEVPVNWCPA LGTVLANEEV INGRSERGGH
     PVIRKPMRQW MLKITAYADR LLEDLEELDW PESIKEMQRN WIGRSEGAEI EFAVHGHDET
     FTVFTTRPDT LFGATYTVLA PEHPLVEKIT TPEQKPAVDA YLKEIQSKSD LERTDLAKEK
     TGVFTGAYAI HPVTGDRLPI WIADYVLMSY GTGAIMAVPA HDERDYEFAK KFHLPMKEVV
     AGGDIEKEPY TGDGEHINSE FLNGLNKQEA IDKMIAWLEE HGKGRKKVSY RLRDWLFSRQ
     RYWGEPIPII HWEDGTMTPV PEEELPLVLP KTDEIRPSGT GESPLANIEE WVNVVDPKTG
     KKGRRETNTM PQWAGSCWYY LRYIDPHNDK QLADPEKLKK WLPVDVYIGG AEHAVLHLLY
     ARFWHKFLYD LGIVPTKEPF QKLFNQGMIL GENNEKMSKS KGNVVNPDDI IESHGADTLR
     LYEMFMGPLE ASIAWSTKGL DGARRFLDRV WRLFVTENGE LNPNIVDEPA NDTLERIYHQ
     TVKKVTEDYE SLRFNTAISQ LMVFINEAYK AEQMKKEYME GFVKLLSPVC PHIGEELWQK
     LGHTDTIAYE PWPTYDETKL VEDVVEIVVQ INGKVRSRLH VPVDLPKEAL EERALADEKI
     KEQLEGKTVR KVIAVPGKLV NIVAN