ID   BLN03_BRELC             Reviewed;         169 AA.
AC   A0A3F2YED3;
DT   18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2019, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Secreted RxLR effector protein BLN03 {ECO:0000303|PubMed:30251420};
DE   Flags: Precursor;
GN   Name=BLN03 {ECO:0000303|PubMed:30251420}; ORFNames=CCR75_006621;
OS   Bremia lactucae (Lettuce downy mildew).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Bremia.
OX   NCBI_TaxID=4779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DOMAIN.
RC   STRAIN=Race Bl:24;
RX   PubMed=30251420; DOI=10.1111/mpp.12751;
RA   Pelgrom A.J.E., Eikelhof J., Elberse J., Meisrimler C.N., Raedts R.,
RA   Klein J., Van den Ackerveken G.;
RT   "Recognition of lettuce downy mildew effector BLR38 in Lactuca serriola
RT   LS102 requires two unlinked loci.";
RL   Mol. Plant Pathol. 20:240-253(2019).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SF5;
RA   Fletcher K.;
RT   "Genomic signatures of somatic hybrid vigor due to heterokaryosis in the
RT   oomycete pathogen, Bremia lactucae.";
RL   Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION.
RX   PubMed=31077456; DOI=10.1111/tpj.14383;
RA   Meisrimler C.N., Pelgrom A.J.E., Oud B., Out S., Van den Ackerveken G.;
RT   "Multiple downy mildew effectors target the stress-related NAC
RT   transcription factor LsNAC069 in lettuce.";
RL   Plant J. 99:1098-1115(2019).
CC   -!- FUNCTION: Secreted effector that inhibits stress-induced relocalization
CC       of the endoplasmic reticulum tail-anchored transcription factors to the
CC       nucleus, thus affecting stress responses.
CC       {ECO:0000305|PubMed:31077456}.
CC   -!- SUBUNIT: Interacts with host transcription factor NAC069.
CC       {ECO:0000269|PubMed:31077456}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31077456}. Host
CC       membrane {ECO:0000305|PubMed:31077456}; Single-pass type I membrane
CC       protein {ECO:0000255}.
CC   -!- DOMAIN: Has the canonical EER motif, but lacks the canonical
CC       translocation motif RxLR, which characterizes most oomycete effectors
CC       identified so far. {ECO:0000305|PubMed:30251420}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; MG686575; AYE92119.1; -; mRNA.
DR   EMBL; SHOA01000114; TDH67208.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3F2YED3; -.
DR   VEuPathDB; FungiDB:CCR75_006621; -.
DR   Proteomes; UP000294530; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Host membrane; Membrane; Reference proteome; Secreted; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..169
FT                   /note="Secreted RxLR effector protein BLN03"
FT                   /id="PRO_0000447897"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           51..54
FT                   /note="dEER"
FT                   /evidence="ECO:0000305|PubMed:30251420"
SQ   SEQUENCE   169 AA;  18873 MW;  3489A8D7503D8459 CRC64;
     MRPRKYIVVV LLSIAYTMCL AVGYPSDGTD DSTKSLKSGN RIRSHTPDIG TEERTLTWLK
     SMLRPSSSSA KVTKEIPEVV KSASDFKVRQ TLSAITRKLG LHSQRLKRFA SWLRKTGERL
     TRKKVYYAGY IAIVDSSSSQ LKRLSVTYGS AFLFVIGFIV LLAFAMTAV