SYL_GEOSM
ID SYL_GEOSM Reviewed; 824 AA.
AC C6E100;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-SEP-2009, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=GM21_0766;
OS Geobacter sp. (strain M21).
OC Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC Geobacteraceae; Geobacter; unclassified Geobacter.
OX NCBI_TaxID=443144;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M21;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C.,
RA Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA Lovley D.;
RT "Complete sequence of Geobacter sp. M21.";
RL Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; CP001661; ACT16840.1; -; Genomic_DNA.
DR RefSeq; WP_012774457.1; NC_012918.1.
DR AlphaFoldDB; C6E100; -.
DR SMR; C6E100; -.
DR STRING; 443144.GM21_0766; -.
DR EnsemblBacteria; ACT16840; ACT16840; GM21_0766.
DR KEGG; gem:GM21_0766; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_7; -.
DR OMA; TFMVLAP; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 2.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..824
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000202220"
FT MOTIF 42..52
FT /note="'HIGH' region"
FT MOTIF 581..585
FT /note="'KMSKS' region"
FT BINDING 584
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 824 AA; 92698 MW; 873509B070F041FC CRC64;
MEEKYNPSAV EEKWQGYWAE HESFKATEDP TRKKYYLLEM FPYPSGKIHM GHVRNYSIGD
VIARFKRMQG YNVLHPMGWD AFGMPAENAA IQHKSHPAKW TYENIAYMRG QLKTLGLSYD
WDRELATCDL DYYKWEQRIF LEMYKKGLAY KKSSAVNWCP KCETVLANEQ VEDGCCWRCD
SPVRQKELEQ WSFRITNYAQ ELLDDTYKLT GWPERVLTMQ RNWIGRSTGC EIDFPLENGL
GKIKVFTTRQ DTLFGATFMS LAAEHPMALD LAGDAQRAQV EAFIDKVKKT DRIKRGAEDL
EKEGVFTGSY CVNPVTNTKM PIYLANFVLM DYGTGAVMAV PTHDQRDFEF AKKYNLPLKV
VIQPEGETLD PAAMTEAYTA EGIMANSGRF DGMGNGDAKE AIADFLEKEG IGKKTVNFRL
RDWGISRQRY WGNPIPVINC DLCGVVAVPE ADLPVVLPMD AEFTGEGGNP LARVDSFTTC
TCPQCGEAAR RETDTMDTFV QSSWYFLRYC SPKFSAGPLD REKVEAWMPV DQYIGGIEHA
VLHLLYARFF TKVLRDLGYC NVDEPFSNLL TQGMVIKDGA KMSKSKGNVV DPNALIERYG
ADTARLFSLF AAPPEKDLDW SDQGVDGSYR FLNRVWRLVY DVLPVIGEAG AVNPDSLGAE
AKKLRRAVHK TIKKVSEDVE ERFHFNTAIA AVMELVNAIQ AFAAKDAPEN VAVVREAVES
VVRLLAPFVP HFAEELWSQL GHDTVLEAAG WPGYDAAAVV DEEVTVVIQV NGKLRSKLTV
APDAKEEEVR AQALADDKIK PYLEGKDVKK VVYVPGKLVS IVVA
