SYL_GEOTN
ID SYL_GEOTN Reviewed; 805 AA.
AC A4IRY3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=GTNG_2742;
OS Geobacillus thermodenitrificans (strain NG80-2).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX NCBI_TaxID=420246;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NG80-2;
RX PubMed=17372208; DOI=10.1073/pnas.0609650104;
RA Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X.,
RA Han W., Peng X., Liu R., Wang L.;
RT "Genome and proteome of long-chain alkane degrading Geobacillus
RT thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABO68087.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000557; ABO68087.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_008880943.1; NC_009328.1.
DR AlphaFoldDB; A4IRY3; -.
DR SMR; A4IRY3; -.
DR STRING; 420246.GTNG_2742; -.
DR EnsemblBacteria; ABO68087; ABO68087; GTNG_2742.
DR KEGG; gtn:GTNG_2742; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_9; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000001578; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 2.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..805
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000334760"
FT MOTIF 40..51
FT /note="'HIGH' region"
FT MOTIF 576..580
FT /note="'KMSKS' region"
FT BINDING 579
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 805 AA; 92346 MW; 4C47AD64DB2BD5D9 CRC64;
MSFNHREIEK KWQDYWEQHK TFRTPDESDK PKFYVLDMFP YPSGAGLHVG HPEGYTATDI
LARMKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAEFT QKNIDNFRRQ IKSLGFSYDW
DREINTTDPN YYKWTQWIFL KLYEKGLAYM DEVPVNWCPA LGTVLANEEV INGRSERGGH
PVIRKPMRQW MLKITAYADR LLEDLEELDW PESIKEMQRN WIGRSEGAEI EFAVDGHDES
FTVFTTRPDT LFGATYAVLA PEHPLVEKIT TPEQKPAVDA YLKEVQSKSD LERTDLAKEK
TGVFTGAYAI HPVTGDKLPI WIADYVLMGY GTGAIMAVPA HDERDYEFAK TFNLPIKEVV
AGGNVENEPY TGDGEHINSE FLNGLNKQEA IEKMIAWLEE NGKGQKKVSY RLRDWLFSRQ
RYWGEPIPVI HWEDGTMTTV PEEELPLVLP KTDEIKPSGT GESPLANIEE WVNVVDPKTG
KKGRRETNTM PQWAGSCWYY LRYIDPHNDK QLADPEKLKQ WLPVDVYIGG AEHAVLHLLY
ARFWHKVLYD LGIVPTKEPF QKLFNQGMIL GENNEKMSKS KGNVVNPDDI VESHGADTLR
LYEMFMGPLE ASIAWSTKGL DGARRFLERV WRLFVTEDGQ LNPNIVDEPA NDTLERVYHQ
TVKKVTEDYE ALRFNTAISQ LMVFINEAYK AEQMKKEYME GFVKLLSPVC PHIGEELWQK
LGHTDTIAYE PWPTYDEAKL VEDVVEIVIQ INGKVRAKLN VPADLSKEAL EERALADEKI
KEQLAGKTVR KVITVPGKLV NIVAN
