ID   BLN04_BRELC             Reviewed;         147 AA.
AC   A0A3B7TNF2;
DT   18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2019, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Secreted RxLR effector protein BLN04 {ECO:0000303|PubMed:30251420};
DE   Flags: Precursor;
GN   Name=BLN04 {ECO:0000303|PubMed:30251420};
OS   Bremia lactucae (Lettuce downy mildew).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Bremia.
OX   NCBI_TaxID=4779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DOMAIN.
RC   STRAIN=Race Bl:24;
RX   PubMed=30251420; DOI=10.1111/mpp.12751;
RA   Pelgrom A.J.E., Eikelhof J., Elberse J., Meisrimler C.N., Raedts R.,
RA   Klein J., Van den Ackerveken G.;
RT   "Recognition of lettuce downy mildew effector BLR38 in Lactuca serriola
RT   LS102 requires two unlinked loci.";
RL   Mol. Plant Pathol. 20:240-253(2019).
RN   [2]
RP   INTERACTION WITH NAC069, AND FUNCTION.
RX   PubMed=31077456; DOI=10.1111/tpj.14383;
RA   Meisrimler C.N., Pelgrom A.J.E., Oud B., Out S., Van den Ackerveken G.;
RT   "Multiple downy mildew effectors target the stress-related NAC
RT   transcription factor LsNAC069 in lettuce.";
RL   Plant J. 99:1098-1115(2019).
CC   -!- FUNCTION: Secreted effector that inhibits stress-induced relocalization
CC       of the transcription factor NAC069 to the nucleus, thus affecting its
CC       broad role in abiotic and biotic stress responses.
CC       {ECO:0000269|PubMed:31077456}.
CC   -!- SUBUNIT: Interacts with host transcription factor NAC069.
CC       {ECO:0000269|PubMed:31077456}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31077456}. Host
CC       membrane {ECO:0000305|PubMed:31077456}; Single-pass type I membrane
CC       protein {ECO:0000255}.
CC   -!- DOMAIN: Has the canonical EER motif, but lacks the canonical
CC       translocation motif RxLR, which characterizes most oomycete effectors
CC       identified so far. {ECO:0000305|PubMed:30251420}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; MG686576; AYE92120.1; -; mRNA.
DR   AlphaFoldDB; A0A3B7TNF2; -.
DR   VEuPathDB; FungiDB:CCR75_008813; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Host membrane; Membrane; Secreted; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..147
FT                   /note="Secreted RxLR effector protein BLN04"
FT                   /id="PRO_5017692030"
FT   TRANSMEM        117..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           58..61
FT                   /note="dEER"
FT                   /evidence="ECO:0000305|PubMed:30251420"
SQ   SEQUENCE   147 AA;  16461 MW;  E77F61D6DA86243E CRC64;
     MATMRRICFL FVFNLAVATS TQGISDEISP DIPETSQAVI DGHRLKGSSK VSKATTGSEE
     RFIRSQLQFL INKIFGARGV AKVLVEEGPK SGVFNKLKEI LVKISKFEMK GDMLYMVYIY
     TILFLSIPII LGVAMYINHH VESSYIH