ID   SYL_HALOH               Reviewed;         830 AA.
AC   B8CXR8;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Hore_13370;
OS   Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562).
OC   Bacteria; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae;
OC   Halothermothrix.
OX   NCBI_TaxID=373903;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H 168 / OCM 544 / DSM 9562;
RX   PubMed=19145256; DOI=10.1371/journal.pone.0004192;
RA   Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H.,
RA   Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C.;
RT   "Genome analysis of the anaerobic thermohalophilic bacterium
RT   Halothermothrix orenii.";
RL   PLoS ONE 4:E4192-E4192(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP001098; ACL70087.1; -; Genomic_DNA.
DR   RefSeq; WP_012636271.1; NC_011899.1.
DR   AlphaFoldDB; B8CXR8; -.
DR   SMR; B8CXR8; -.
DR   STRING; 373903.Hore_13370; -.
DR   PRIDE; B8CXR8; -.
DR   EnsemblBacteria; ACL70087; ACL70087; Hore_13370.
DR   KEGG; hor:Hore_13370; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_9; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000000719; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 2.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..830
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000199209"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           585..589
FT                   /note="'KMSKS' region"
FT   BINDING         588
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   830 AA;  96269 MW;  9BB05557D27E2CE8 CRC64;
     MKGYYNFADI EKKWQDKWEK DGLYKTQEET DKDNYYVLEM FPYPSGNLHM GHVRVYSIGD
     VIARFKRMNG YNVLHPMGWD AFGLPAENAA IKHGNIHPQD WTWDNIKNMK KQMKSLGLSY
     DWDREVTTAK EDYYKWTQWF FVKMFKKGLA YKKKAAVNWC PGCETVLANE QVVNNACERC
     GTEVEEKELE QWFFKITNYA ERLLEDHKLL QNWPEKVKIM QRNWIGRSEG MRIKFPVKGS
     SEEIEVFTTR PDTIFGATYM VLAPEHPLVE KLISGTEKEK EVRQFIDRVK KQKEMERTSP
     ESEKEGLFTG AYAINPMTGE EIPIMIANYV LMGYGTGAIM AVPAHDQRDF DFARKYDLPI
     RVVIQPEERE EELKDTDLNE AYEGSGHLIN SDKYNGLTVK EAFDVMAEDM EKEGIGKREV
     NYRLRDWLIS RQRYWGTPIP IVYCEKCGTV PVPEEELPVV LPRDVEFSPT GESPLAKVDE
     FVNTTCPVCG GKARRETDTM DTFVDSSWYF LRYTDPKNDK LPFSKENAKK WFPVDQYIGG
     IEHAILHLLY ARFFTKVIYD MDMIDSVEPF TNWLAQGMVL KDGAKMSKSK GNVVDPEDIL
     DRFGADTARL FILFAAPPEK DLEWSDRGVE GAERFLNRVW RLVADNIKEI KNTDQASLDV
     NSFNKNEKDL YRNLHVTIKR VTEDIGERLN FNTAISAIME LTNATYQYLN GVEKVNYTLI
     KDIIEKMLLI LAPFAPHMTE ELWSELGNDE SIHIQKWPGY EEKALKKDEV TIVVQVNGKV
     RDKLQVSADI DEDKLKEQVL ELPRIQKYTE GKEIVKTIII PKKLVNIVVK