ID   BLN06_BRELC             Reviewed;         502 AA.
AC   A0A3B7TLV2;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2019, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=RxLR effector protein BLN06 {ECO:0000303|PubMed:30251420};
DE   Flags: Precursor;
GN   Name=BLN06 {ECO:0000303|PubMed:30251420};
OS   Bremia lactucae (Lettuce downy mildew).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Bremia.
OX   NCBI_TaxID=4779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], DOMAIN, FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=Race Bl:24;
RX   PubMed=30251420; DOI=10.1111/mpp.12751;
RA   Pelgrom A.J.E., Eikelhof J., Elberse J., Meisrimler C.N., Raedts R.,
RA   Klein J., Van den Ackerveken G.;
RT   "Recognition of lettuce downy mildew effector BLR38 in Lactuca serriola
RT   LS102 requires two unlinked loci.";
RL   Mol. Plant Pathol. 20:240-253(2019).
CC   -!- FUNCTION: Secreted effector that triggers a robust hypersensitive
CC       response (HR) in Lactuca serriola LS102. The response to BLN06 was
CC       visible as chlorosis but not as strong necrosis.
CC       {ECO:0000269|PubMed:30251420}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30251420}. Host cell
CC       membrane {ECO:0000269|PubMed:30251420}.
CC   -!- DOMAIN: Has the canonical EER motif, but lacks the canonical
CC       translocation motif RxLR, which characterizes most oomycete effectors
CC       identified so far. {ECO:0000305|PubMed:30251420}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; MG686578; AYE92122.1; -; mRNA.
DR   AlphaFoldDB; A0A3B7TLV2; -.
DR   SMR; A0A3B7TLV2; -.
DR   VEuPathDB; FungiDB:CCR75_008226; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Host cell membrane; Host membrane; Membrane; Secreted;
KW   Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..502
FT                   /note="RxLR effector protein BLN06"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5017662402"
FT   MOTIF           50..53
FT                   /note="dEER"
FT                   /evidence="ECO:0000305|PubMed:30251420"
FT   CARBOHYD        38
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   502 AA;  57204 MW;  28104D0E51E7CDA0 CRC64;
     MTLLHCWLLL VGHLASTAYA DFITKDFKSL PPPAYDTNAT QALVPYNAAL EERNGPSSST
     ALLQYIDHKP GLMKKLLAGL SIRFAPPTMK VISTPTDMLR IDKIKNNIIK SSQWKRWARS
     LLEQNSMQNS HVIITKKMMD DLKPTNFFLV LLEASKDKNT KAVAKILEET QFARWCTIEH
     ESISPMEFYD VLQLNLNEPI AYMERLPVLL RYWKYYKSVH SPMSSVATPK DIIDKQTVNR
     FGPYWKHTGE IAELLRLDFD SDSFFNHPAR NVWLDLMKTY LDDTKTAEPL MIKTFQLLGN
     AAAKNLQNNV YSPIHFAERW IQANLQPIDV VTILGLDIHD SNLATNSAFS FLKVFIEKFL
     VNHPEADTTV VKIFSRLGSD ESEKALALRK SFVSFFLRTP KFTPKTVMSI FDLTISADYV
     EKNPVWAIWM EYVNVYLVKN KVCPEGPLAD TLEFLGSTAA ADGVVRKKSI ELLYSSWSGK
     TSQDTRIKQF LTAAARLNQL EL