ID   SYL_LACH4               Reviewed;         804 AA.
AC   A8YWU4;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=lhv_1726;
OS   Lactobacillus helveticus (strain DPC 4571).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=405566;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DPC 4571;
RX   PubMed=17993529; DOI=10.1128/jb.01295-07;
RA   Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K.,
RA   McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F.,
RA   Beresford T., Ross R.P.;
RT   "Genome sequence of Lactobacillus helveticus: an organism distinguished by
RT   selective gene loss and IS element expansion.";
RL   J. Bacteriol. 190:727-735(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000517; ABX27605.1; -; Genomic_DNA.
DR   RefSeq; WP_003627282.1; NC_010080.1.
DR   AlphaFoldDB; A8YWU4; -.
DR   SMR; A8YWU4; -.
DR   STRING; 405566.lhv_1726; -.
DR   EnsemblBacteria; ABX27605; ABX27605; lhv_1726.
DR   KEGG; lhe:lhv_1726; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_9; -.
DR   OMA; TFMVLAP; -.
DR   Proteomes; UP000000790; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 2.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..804
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000091328"
FT   MOTIF           39..50
FT                   /note="'HIGH' region"
FT   MOTIF           573..577
FT                   /note="'KMSKS' region"
FT   BINDING         576
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   804 AA;  92396 MW;  430DC21235A47AEB CRC64;
     MYNHKVVEKK WQDYWAKHDT FKTGTDPKKK NYYALDMFPF PSGKGLHVGH PEGYTATDIV
     SRMKRAQGYN VLHPMGWDAF GLPTEQYALK TGEDPAVVTK NNIANFKRQL NKLGFSYDWD
     REITTSDPNY YKWTQWVFEQ MYKKGLAYEA EVPVNWSPDL GTVVANEEVI DGKTERGGYP
     VYRRNMRQWM LKMTAYADRL LEDLDDLDWP EPVKEMQRNW IGRSVGAQVT FKIKDSDKTF
     DIFTTRPDTL FGCSYTVLAP ENKLVQEITT DGHRDEVNAY IKKIESKSDL ERTDLNKDKT
     GVFTGAYAIN PVNGKEVPIW ISDYVLASYG TGAVMAVPAH DDRDYAFAKK FDLPINQVLE
     GGDLSKAAFT EDGPHINSEF LNGLNIKDAK KKMVDWLEEH NCGEKKVNYK LRDWDFSRQR
     YWGEPIPVIH WEDGTTSLVP EDQLPLRLPH ATDIKPSGTP ESPLANLTDW VNVVDEMGRK
     GKRETNTMPN WAGSSWYYLR YVDPHNDKEL ADYDLLKQWL PVDLYIGGAE HAVRHLLYAR
     FWHKVLYDLG VVPTKEPFQR LYNQGLILKN HEKMSKSKGN VVNPDEVIDE YGADSLRMYE
     MFMGPLDASI DWDDNGPAST KKFLDRVWRL FVNDLDLKAI PQERIVDKND GELDKVYAET
     VKKVTEDFDA LHFNTAISQM MVFINAAQKA KTIPREYVEG FVKLLAPVAP HMMEEIWQVF
     GHDESITYAK WPTYDPAKLV ESTVEIMVQV NGKLRGKFEA AKDADRDDVQ KQAMALPHVQ
     KFLEGKDVKK VIVVPNKIVN IVAK