ID   SYL_METFK               Reviewed;         817 AA.
AC   Q1GZB5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2006, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Mfla_2155;
OS   Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Methylophilaceae; Methylobacillus.
OX   NCBI_TaxID=265072;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KT / ATCC 51484 / DSM 6875;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.;
RT   "Complete sequence of Methylobacillus flagellatus KT.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000284; ABE50422.1; -; Genomic_DNA.
DR   RefSeq; WP_011480376.1; NC_007947.1.
DR   AlphaFoldDB; Q1GZB5; -.
DR   SMR; Q1GZB5; -.
DR   STRING; 265072.Mfla_2155; -.
DR   PRIDE; Q1GZB5; -.
DR   EnsemblBacteria; ABE50422; ABE50422; Mfla_2155.
DR   KEGG; mfa:Mfla_2155; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_4; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000002440; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..817
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009372"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           576..580
FT                   /note="'KMSKS' region"
FT   BINDING         579
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   817 AA;  91608 MW;  FE410E4C7D9E64DD CRC64;
     MQQQYPFRDI EQQAQQYWES NATFTATEVS DKPKYYCLSM FPYPSGKLHM GHVRNYTIGD
     VLSRFHRMRG YNVMQPMGWD AFGLPAENAA IKHNVAPAGW TYSNIEHMKG QLKSLGLAVD
     WQREIATCKP DYYRWEQWLF TELYKKGLIY KKTATVNWDP VDQTVLANEQ VIDGRGWRSG
     ALVEKRDIPQ YFMKITAYAE ELLADLDKLD GWPEQVKTMQ RNWIGKSYGC EVEFPLAVGN
     GTLKVYTTRP DTLMGATYVA IAAEHALATQ AAKDNPALQA FIEECKRGSV AEADLATAEK
     KGMNSGLFVL HPITGEKLPV WVANYVLISY GEGAVMAVPA HDERDFEFAS KYKLPIKAVI
     KPAHGELELP LQAAYTEHGI LFDSGDFNGL DFSQASDAIA AALAAKNLGK RRTQYRLRDW
     GISRQRYWGC PIPIVHCPSC GEVPVPAEQL PVVLPEDVVM DGVGSPIKKD PAFYETTCPS
     CGEKATRETD TMDTFVESSW YFARYASFDA HSSMVDERAN YWLPVDQYIG GIEHAILHLL
     YARFFNKLMR DVGLVRHDEP FTKLLTQGMV LKDGSKMSKS KGNTVDPQEL IDNYGADTAR
     LFMMFAAPPE QSLEWSDAGV EGAHRFLKRV WTAVANHVES GIVAAFTMGD LSAELKAFRR
     QLHQTIEKVT DDYGRRHAFN TAIAAVMELM NAYAKIEGHD ATTRAIRQEA LENIILLLSP
     IVPHICHALW QALRPGTQLL DTRWPVANKA AMVQDEIELV LQVNGKLRGS MTVSRTLDKA
     AIEALAVQQE CIQKYLAEGS VRKIIVVPNK LVNIVVG