ID   SYL_METMA               Reviewed;         966 AA.
AC   Q8Q054;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 2.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=MM_0283;
OS   Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS   11833 / OCM 88) (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=192952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT   between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM29979.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE008384; AAM29979.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_048038036.1; NC_003901.1.
DR   AlphaFoldDB; Q8Q054; -.
DR   SMR; Q8Q054; -.
DR   STRING; 192952.MM_0283; -.
DR   PRIDE; Q8Q054; -.
DR   EnsemblBacteria; AAM29979; AAM29979; MM_0283.
DR   GeneID; 44086522; -.
DR   KEGG; mma:MM_0283; -.
DR   PATRIC; fig|192952.21.peg.347; -.
DR   eggNOG; arCOG00809; Archaea.
DR   HOGENOM; CLU_004174_0_0_2; -.
DR   OMA; AWNMAFQ; -.
DR   Proteomes; UP000000595; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 1.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_A; Leu_tRNA_synth_A; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR020791; Leu-tRNA-lgase_arc.
DR   InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR45794; PTHR45794; 2.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 2.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00395; leuS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..966
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000152133"
FT   MOTIF           41..51
FT                   /note="'HIGH' region"
FT   MOTIF           632..636
FT                   /note="'KMSKS' region"
FT   BINDING         635
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   966 AA;  110957 MW;  561D0FDC574A0DC3 CRC64;
     MEQDYKPHEI ENKWQKKWNE SRIFQAEPDK REKFFITIPY PYLNGNLHAG HTRTFTIGDV
     VARHKRMLGY NVLYPMGFHV TGTPIVGLAE LIASRDPQTM DVYERLHGIP GDILPALDTP
     EKIVDYFKVE AEKAMRMIGY SIDWRRKFTT TDPTYKKFIE WQYTRLEEKD LIVKGSHPVK
     WCPNDNNPVE DHDILHGEEA TIVEYTLIKF RYRDLVLPCA TLRPETTYGV TNLWVNPNVD
     YVKARVEKDG NVEFWVVSRD AFRKLTFTDR TVEYVEDMPA KSIIGIKLTN PVTGDEVISL
     PASFVKPENG SGIVMSVPAH APFDYLALRD LYDADLSEYG ITEDLRKIEL ISLIQVPEFG
     EFPAKEIVES MGISDQKDPK AEEATKIVYR REFHGGVLKE LTGKYKGYPV SKIKDILTRD
     FLASNAGETF YEFSEPVVCR CGTPCVVNMV KGQWFLNYSN PDWKAKVYKC LGQMRVIPTE
     YRVEFENKID WLKDKACARR KGLGTRLPFD KEWLIESLGD STIYMSYYII ARFIEKGELA
     LEHLTLSFFD YVLLGKGDSA AVSAETGLKP ELIEEIRSHF NYWYPVDLRS SGKDLVPNHL
     LFFLFHHVAL FEEEKWPKAL AVNGFVSLEG QKMSKSKGPI LTLESAVSSY GADITRMYIL
     STAEQTQDAD WQKAGIDSAR RQVDRFYAFA KDVIESGKRG TLSAELKQID RWMLSRMQNY
     IKETNAALDS IQTREAIQNS FFLLINDVRW YQRRGGEALL YYVLDNWVRL MAPFTPHLCE
     EVWEAMGHED PVSLAQYPLY NEDLIDNGAE LSEEAVKSTL NDIEEIIRVT KMTPQKVYLY
     TAPAWKAEAI RCACGLQVEA PLEVGTLIKT LMAKPELKRF GKEIPKFVQK IVPEFKSGGA
     ERYETFAYLG LDEKDLLKES ASFLEKEIGC PVEIQSADSP EYDPQKKSRF AEPLRPAIYI
     EEKKEE