SYL_MYCBO
ID SYL_MYCBO Reviewed; 969 AA.
AC P67511; A0A1R3XU37; P71698; X2BDV0;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049};
GN OrderedLocusNames=BQ2027_MB0042;
OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=233413;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT "The complete genome sequence of Mycobacterium bovis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA Robbe-Austerman S., Gordon S.V.;
RT "Updated reference genome sequence and annotation of Mycobacterium bovis
RT AF2122/97.";
RL Genome Announc. 5:E00157-E00157(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; LT708304; SIT98402.1; -; Genomic_DNA.
DR RefSeq; NP_853711.1; NC_002945.3.
DR RefSeq; WP_003900794.1; NC_002945.4.
DR AlphaFoldDB; P67511; -.
DR SMR; P67511; -.
DR EnsemblBacteria; SIT98402; SIT98402; BQ2027_MB0042.
DR GeneID; 45424000; -.
DR PATRIC; fig|233413.5.peg.48; -.
DR OMA; TFMVLAP; -.
DR Proteomes; UP000001419; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 3.
DR Gene3D; 3.90.740.10; -; 1.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..969
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000152043"
FT MOTIF 78..89
FT /note="'HIGH' region"
FT MOTIF 739..743
FT /note="'KMSKS' region"
FT BINDING 742
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 969 AA; 107595 MW; A07C835FC1B428D8 CRC64;
MTESPTAGPG GVPRADDADS DVPRYRYTAE LAARLERTWQ ENWARLGTFN VPNPVGSLAP
PDGAAVPDDK LFVQDMFPYP SGEGLHVGHP LGYIATDVYA RYFRMVGRNV LHALGFDAFG
LPAEQYAVQT GTHPRTRTEA NVVNFRRQLG RLGFGHDSRR SFSTTDVDFY RWTQWIFLQI
YNAWFDTTAN KARPISELVA EFESGARCLD GGRDWAKLTA GERADVIDEY RLVYRADSLV
NWCPGLGTVL ANEEVTADGR SDRGNFPVFR KRLRQWMMRI TAYADRLLDD LDVLDWPEQV
KTMQRNWIGR STGAVALFSA RAASDDGFEV DIEVFTTRPD TLFGATYLVL APEHDLVDEL
VAASWPAGVN PLWTYGGGTP GEAIAAYRRA IAAKSDLERQ ESREKTGVFL GSYAINPANG
EPVPIFIADY VLAGYGTGAI MAVPGHDQRD WDFARAFGLP IVEVIAGGNI SESAYTGDGI
LVNSDYLNGM SVPAAKRAIV DRLESAGRGR ARIEFKLRDW LFARQRYWGE PFPIVYDSDG
RPHALDEAAL PVELPDVPDY SPVLFDPDDA DSEPSPPLAK ATEWVHVDLD LGDGLKPYSR
DTNVMPQWAG SSWYELRYTD PHNSERFCAK ENEAYWMGPR PAEHGPDDPG GVDLYVGGAE
HAVLHLLYSR FWHKVLYDLG HVSSREPYRR LVNQGYIQAY AYTDARGSYV PAEQVIERGD
RFVYPGPDGE VEVFQEFGKI GKSLKNSVSP DEICDAYGAD TLRVYEMSMG PLEASRPWAT
KDVVGAYRFL QRVWRLVVDE HTGETRVADG VELDIDTLRA LHRTIVGVSE DFAALRNNTA
TAKLIEYTNH LTKKHRDAVP RAAVEPLVQM LAPLAPHIAE ELWLRLGNTT SLAHGPFPKA
DAAYLVDETV EYPVQVNGKV RGRVVVAADT DEETLKAAVL TDEKVQAFLA GATPRKVIVV
AGRLVNLVI
