ID   SYL_NITEU               Reviewed;         869 AA.
AC   Q820M7;
DT   07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=NE1139;
OS   Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC
OS   14298).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=228410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298;
RX   PubMed=12700255; DOI=10.1128/jb.185.9.2759-2773.2003;
RA   Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L.,
RA   Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A.,
RA   Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.;
RT   "Complete genome sequence of the ammonia-oxidizing bacterium and obligate
RT   chemolithoautotroph Nitrosomonas europaea.";
RL   J. Bacteriol. 185:2759-2773(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; AL954747; CAD85050.1; -; Genomic_DNA.
DR   RefSeq; WP_011111730.1; NC_004757.1.
DR   AlphaFoldDB; Q820M7; -.
DR   SMR; Q820M7; -.
DR   STRING; 228410.NE1139; -.
DR   EnsemblBacteria; CAD85050; CAD85050; NE1139.
DR   KEGG; neu:NE1139; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_4; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   PhylomeDB; Q820M7; -.
DR   Proteomes; UP000001416; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 2.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..869
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000152055"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           624..628
FT                   /note="'KMSKS' region"
FT   BINDING         627
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   869 AA;  98496 MW;  4A9387EE8921BC96 CRC64;
     MQEKYHPQEI ERQARQSWQE TNIFNVTEIP DRPKYYCLSM FPYPSGKLHM GHVRNYTIGD
     VLSRYRRMQG YNVMQPMGWD AFGLPAENAA IQKGVPPAKW TYDNIAYMRS QLQSLGFAID
     WQRELATCDP QYYRWNQWLF LRMLEKGIAY QKTQVVNWDP VDQTVLANEQ VIDGCGWRTG
     AVVEKREIPG YYLAITRYAD ELLADLEKLP GWPERVKTMQ ANWIGKSFGV DITFPPDTAS
     GMPQALKVFT TRADTLMGVT YVAVAAEHPV ALHAAHNQPD LVAFIESCRQ GATMEAELAV
     QEKKGMATGL YVLHPLTGER LPVWVANYVL MSYGEGAVMA VPAHDERDFD FARQHSLPIK
     PVIRPENGEL SVPLVQAFTE YGVTFNSDRF SDLTSPEAID AIAVELGQKA LGEKRVRYRL
     RDWGISRQRY WGCPIPLIHC DSCGVVPVAD DQLPVVLPED LVPDGSGNPL AKTPSFYECT
     CPRCGRQARR ETDTMDTFVD SSWYFIRYAC PDQSAAMTDQ RANYWLPVDQ YIGGIEHAIL
     HLLYSRFWSK VMRDLGLVSF DEPFANLLTQ GMVLNEIFFR KTSSGRIQYF NPAEVDVQHD
     GEGKRVGAVL QADNQPVESG GIGTMSKSKN NGIDPQEIIE QYGADTARLF MMFASPPTQT
     LEWSDAGVEG AFRFLKRLWR QVYLHRQLDG EAAATTTLVP HQEYPADLRD LRCQLHQTIV
     KVTDDLERRH TFNTAIAAIM ELMNELSDVQ GTHPAARQLM QEALENIVLL LSPIVPHICH
     VLWRELRPGT ELLDQPWPQA DDQALIQDEV EIVVQINGKL RGQIRIAREA DRAAVERTAL
     QDEHIQKSIA YRPVKRVIVV PGKLINIVV