ID   SYL_NOSP7               Reviewed;         871 AA.
AC   B2J7T9;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Npun_F4156;
OS   Nostoc punctiforme (strain ATCC 29133 / PCC 73102).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=63737;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29133 / PCC 73102;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C., Elhai J.,
RA   Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.;
RT   "Complete sequence of chromosome of Nostoc punctiforme ATCC 29133.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP001037; ACC82534.1; -; Genomic_DNA.
DR   RefSeq; WP_012410501.1; NC_010628.1.
DR   AlphaFoldDB; B2J7T9; -.
DR   SMR; B2J7T9; -.
DR   STRING; 63737.Npun_F4156; -.
DR   PRIDE; B2J7T9; -.
DR   EnsemblBacteria; ACC82534; ACC82534; Npun_F4156.
DR   KEGG; npu:Npun_F4156; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_3; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   PhylomeDB; B2J7T9; -.
DR   Proteomes; UP000001191; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 3.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..871
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000091339"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           634..638
FT                   /note="'KMSKS' region"
FT   BINDING         637
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   871 AA;  98573 MW;  F57D19EF68173BAF CRC64;
     MDSRYNPAAI EEKRQKTWLE LGLDKTPSAS NKPKFYALSM FPYPSGSLHM GHVRNYTITD
     VIARFKRMQG YRVIHPMGWD AFGLPAENAA IDRGVPPAKW TYQNITQMRQ QLQRLGLSID
     WECELATCSP DYYKWTQWIF LQFLQAGLAY QKEAAVNWDP IDQTVLANEQ VDNEGRSWRS
     GAIVERKLLR QWFFKITDYA EELLNDLDKL TGWPERVKLM QANWIGKSTG AYLEFPIVGI
     DEKIAVYTTR PDTVYGVSYL VLAPEHPLTN RVTTKEQQAA VEVFIKEVSN QSELERTSED
     KPKRGIPTGG VAINPFTGEE VPIWIADYVL YEYGTGAVMG VPAHDVRDFK FAKNYDLPIN
     FVIASPDDVA GFDLTPTSEI DGITQLVEVD YKQAYTEPGI LINSGAFTGI SSTDAKQAII
     EYAEKQDFGK VRVQYRLRDW LISRQRYWGA PIPVIHCPNC GIVPVPDKDL PVQLPEEVEF
     TGRCGSPLTQ LESWVNVPCP TCGTPAKRET DTMDTFIDSS WYFLRFPDAK NEQQVFDSSK
     VNDWMPVNQY VGGIEHAILH LLYSRFFTKV LRDRGLLNFD EPFQRLLTQG MVQGLTYLNP
     NKGGKDKWIP SNLVNSADPR DPQTDEPLQR LYATMSKSKG NGVAPEDVIS KYGIDTARMF
     ILFKAPPEKD LEWDEADVEG QFRFLNRVWR LVTDYITAGV SRKKAQSDLT KAEKELRRAI
     HTAIQAVTED VEDEYQFNTA ISELMKLSNA LSDADKNSPI YAEGIRTLVI LIAPFAPHIA
     DELWHLLGES DSIHTQTWPS FDPAALVADE ITLVIQVMGK TRGAIQVPAQ ADKAALEKYA
     RESEIAQRYI EGKEIKKVIV VPGKLVNFVV S