ID   BLO2_KLEOX              Reviewed;         290 AA.
AC   P23954; P71418;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Beta-lactamase OXY-2;
DE            EC=3.5.2.6;
DE   AltName: Full=Penicillinase;
DE   Flags: Precursor;
GN   Name=bla;
OS   Klebsiella oxytoca.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SL911;
RX   PubMed=8834898; DOI=10.1128/aac.40.2.460;
RA   Fournier B., Lagrange P.H., Philippon A.;
RT   "Beta-lactamase gene promoters of 71 clinical strains of Klebsiella
RT   oxytoca.";
RL   Antimicrob. Agents Chemother. 40:460-463(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SB23;
RX   PubMed=8878568; DOI=10.1128/aac.40.9.1988;
RA   Kimura K., Arakawa Y., Ohsuka S., Ito H., Suzuki K., Kurokawa H., Kato N.,
RA   Ohta M.;
RT   "Molecular aspects of high-level resistance to sulbactam-cefoperazone in
RT   Klebsiella oxytoca clinical isolates.";
RL   Antimicrob. Agents Chemother. 40:1988-1994(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=KH11;
RX   PubMed=10223957; DOI=10.1128/aac.43.5.1294;
RA   Wu S.W., Dornbusch K., Kronvall G.;
RT   "Genetic characterization of resistance to extended-spectrum beta-lactams
RT   in Klebsiella oxytoca isolates recovered from patients with septicemia at
RT   hospitals in the Stockholm area.";
RL   Antimicrob. Agents Chemother. 43:1294-1297(1999).
RN   [4]
RP   PROTEIN SEQUENCE OF 28-290.
RC   STRAIN=D488;
RX   PubMed=1909282; DOI=10.1016/0378-1097(91)90301-p;
RA   Reynaud A., Peduzzi J., Barthelemy M., Labia R.;
RT   "Cefotaxime-hydrolysing activity of the beta-lactamase of Klebsiella
RT   oxytoca D488 could be related to a threonine residue at position 140.";
RL   FEMS Microbiol. Lett. 65:185-192(1991).
RN   [5]
RP   PROTEIN SEQUENCE OF 28-290.
RC   STRAIN=HB60;
RX   PubMed=9462429; DOI=10.1093/jac/40.6.789;
RA   Farzaneh S., Peduzzi J., Sofer L., Reynaud A., Barthelemy M., Labia R.;
RT   "Characterization and amino acid sequence of the OXY-2 group beta-lactamase
RT   of pI 5.7 isolated from aztreonam-resistant Klebsiella oxytoca strain
RT   HB60.";
RL   J. Antimicrob. Chemother. 40:789-795(1997).
CC   -!- FUNCTION: Hydrolyzes broad-spectrum beta-lactam antibiotics. Active
CC       against all third-generation cephalosporins but ceftazidime.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC         Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC         ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10101};
CC   -!- MISCELLANEOUS: In strain KH11 OXY-2 is known as OXY-2a.
CC   -!- SIMILARITY: Belongs to the class-A beta-lactamase family.
CC       {ECO:0000305}.
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DR   EMBL; Z49084; CAA88908.1; -; Genomic_DNA.
DR   EMBL; D84548; BAA12695.1; -; Genomic_DNA.
DR   EMBL; Y17714; CAB42614.1; -; Genomic_DNA.
DR   RefSeq; WP_004099854.1; NZ_NDXV01000004.1.
DR   RefSeq; WP_017146441.1; NZ_VKYY01000011.1.
DR   AlphaFoldDB; P23954; -.
DR   SMR; P23954; -.
DR   OrthoDB; 991474at2; -.
DR   GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030655; P:beta-lactam antibiotic catabolic process; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR045155; Beta-lactam_cat.
DR   InterPro; IPR000871; Beta-lactam_class-A.
DR   InterPro; IPR023650; Beta-lactam_class-A_AS.
DR   PANTHER; PTHR35333; PTHR35333; 1.
DR   Pfam; PF13354; Beta-lactamase2; 1.
DR   PRINTS; PR00118; BLACTAMASEA.
DR   SUPFAM; SSF56601; SSF56601; 1.
DR   PROSITE; PS00146; BETA_LACTAMASE_A; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Direct protein sequencing; Hydrolase; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000269|PubMed:1909282,
FT                   ECO:0000269|PubMed:9462429"
FT   CHAIN           28..290
FT                   /note="Beta-lactamase OXY-2"
FT                   /id="PRO_0000016998"
FT   ACT_SITE        72
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10101"
FT   BINDING         236..238
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   VARIANT         15
FT                   /note="Missing (in strain: KH11 and SB23)"
FT   VARIANT         199
FT                   /note="D -> N (in strain: D488 and HB60)"
FT   VARIANT         225
FT                   /note="A -> V (in strain: D488)"
FT   VARIANT         255
FT                   /note="D -> N (in strain: D488)"
SQ   SEQUENCE   290 AA;  31136 MW;  134C4BC24E9999E7 CRC64;
     MIKSSWRKIA MLAAAVPLLL ASGALWASTD AIHQKLTDLE KRSGGRLGVA LINTADNSQI
     LYRGDERFAM CSTSKVMAAA AVLKQSESNK EVVNKRLEIN AADLVVWSPI TEKHLQSGMT
     LAELSAATLQ YSDNTAMNLI IGYLGGPEKV TAFARSIGDA TFRLDRTEPT LNTAIPGDER
     DTSTPLAMAE SLRKLTLGDA LGEQQRAQLV TWLKGNTTGG QSIRAGLPES WVVGDKTGAG
     DYGTTNDIAV IWPEDHAPLV LVTYFTQPQQ DAKNRKEVLA AAAKIVTEGL