ID   SYL_PHOV8               Reviewed;         944 AA.
AC   A6KZH6;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=BVU_1149;
OS   Phocaeicola vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / CCUG 4940 /
OS   NBRC 14291 / NCTC 11154) (Bacteroides vulgatus).
OC   Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC   Phocaeicola.
OX   NCBI_TaxID=435590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8482 / DSM 1447 / JCM 5826 / CCUG 4940 / NBRC 14291 / NCTC
RC   11154;
RX   PubMed=17579514; DOI=10.1371/journal.pbio.0050156;
RA   Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C.,
RA   Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H.,
RA   Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K.,
RA   Knight R.D., Gordon J.I.;
RT   "Evolution of symbiotic bacteria in the distal human intestine.";
RL   PLoS Biol. 5:1574-1586(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000139; ABR38840.1; -; Genomic_DNA.
DR   RefSeq; WP_005849960.1; NC_009614.1.
DR   AlphaFoldDB; A6KZH6; -.
DR   SMR; A6KZH6; -.
DR   STRING; 435590.BVU_1149; -.
DR   PRIDE; A6KZH6; -.
DR   EnsemblBacteria; ABR38840; ABR38840; BVU_1149.
DR   KEGG; bvu:BVU_1149; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_10; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   BioCyc; BVUL435590:G1G59-1196-MON; -.
DR   Proteomes; UP000002861; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 3.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..944
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009294"
FT   MOTIF           40..51
FT                   /note="'HIGH' region"
FT   MOTIF           718..722
FT                   /note="'KMSKS' region"
FT   BINDING         721
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   944 AA;  108274 MW;  90C19E42471E1C73 CRC64;
     MEYNFREIEK KWQQRWAENH TYQVTEDESK KKFYVLNMFP YPSGAGLHVG HPLGYIASDI
     YARYKRLQGF NVLNPMGYDA YGLPAEQYAI QTGQHPAITT VNNINRYREQ LDKIGFSFDW
     NREVRTCEPG YYHWTQWAFQ QMFNSYYCND TQQARPISEL TEAFARYGNE GLNAACSEEL
     SFTAEEWNAK SEKEQQEILM NYRIAYLGET MVNWCPQLGT VLANDEVVDG VSERGGFPVV
     QKKMRQWCLR VSAYAQRLLD GLDTVDWTDS LKETQRNWIG RSEGTEVQFK VKDSDIEFTI
     FTTRADTMFG VTFMVLAPES ELVPQLTTEA QKAEVEAYLD RTKKRTERER IADRRVTGVF
     SGSYAINPFT GEAVPVWISD YVLAGYGTGA IMAVPAHDSR DYAFAKHFNL PIVPLVEGCD
     VSEESFDAKE GIVCNSPRKD VTPYCDLSLN GLTIKEAIAA TKEYVKAHNL GRVKVNYRLR
     DAIFSRQRYW GEPFPVYYKN GMPYMIDSSK LPLELPEVAK FLPTETGEPP LGHATKWAWD
     VEKGEVVENN LIDNVTIFPL ELNTMPGFAG SSAYYLRYMD PHNAQALVSE KADHYWQNVD
     LYVGGTEHAT GHLIYSRFWN KFLHDLGVSI KEEPFQKLVN QGMIQGRSNF VYRIKDTNTF
     VSLNLKDQYE TTPLHVDVNI VSNDILDVEA FKAWRPEYND AEFILEDGKY ICGWAVEKMS
     KSMYNVVNPD MIVEKYGADT LRMYEMFLGP VEQSKPWDTN GIDGVHRFLK KLWNLFYSRT
     DEFLPVEGEP TKEELKAIHK LIKKVTGDIE TFSYNTSISA FMICVNELTS LKCRNKEVLS
     NLIILLAPFA PHYAEELWEA LGNTTSVCDA QWPAFNEDYL KEDTVKYTIS FNGKARFTMD
     FAADADNNTI QTTVMADEQA QKWIEGKTPK KVIIVPKKIV NIVL