SYL_PSE14
ID SYL_PSE14 Reviewed; 868 AA.
AC Q48DN1;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=PSPPH_4394;
OS Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)
OS (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=264730;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1448A / Race 6;
RX PubMed=16159782; DOI=10.1128/jb.187.18.6488-6498.2005;
RA Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R.,
RA Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M.,
RA Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J.,
RA Creasy T., Davidsen T.M., Haft D.H., Zafar N., Zhou L., Halpin R.,
RA Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M.,
RA Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A.,
RA Buell R.;
RT "Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola
RT 1448A reveals divergence among pathovars in genes involved in virulence and
RT transposition.";
RL J. Bacteriol. 187:6488-6498(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; CP000058; AAZ36139.1; -; Genomic_DNA.
DR RefSeq; WP_011169541.1; NC_005773.3.
DR AlphaFoldDB; Q48DN1; -.
DR SMR; Q48DN1; -.
DR STRING; 264730.PSPPH_4394; -.
DR EnsemblBacteria; AAZ36139; AAZ36139; PSPPH_4394.
DR KEGG; psp:PSPPH_4394; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_6; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000000551; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..868
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000009396"
FT MOTIF 42..52
FT /note="'HIGH' region"
FT MOTIF 627..631
FT /note="'KMSKS' region"
FT BINDING 630
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 868 AA; 96868 MW; 6A74904B9092EE52 CRC64;
MHELYQPREI EAAAQTFWDE QKSFEVSEQP GKDTFYCLSM FPYPSGKLHM GHVRNYTIGD
VISRYQRMLG KNVLQPLGWD AFGMPAENAA IDNNVAPAKW TYENIAYMKN QLKSLGLAVD
WSREVTTCKP DYYRWEQWLF TRLFEKGVIY RKNGTVNWDP VDQTVLANEQ VIDGRGWRSG
ALIEKREIPM YYFKITAYAD ELLESLDELP GWPEQVKTMQ RNWIGRSRGM EVQFPYDQAS
IGEAGALKVF TTRPDTLMGA TYVAVAAEHP LATLAAQGNP GLQAFIDECK GGSVAEADVA
TQEKKGQPTS LFVEHPLTGE KLPVWVANYV LMHYGDGAVM AVPAHDERDF EFATHYGLPI
KPVVRTSAGD QTPAPWQPAY GEHGELINSG EFTGLNFQAA FDAIEAALVK KSLGQSRTQF
RLRDWGISRQ RYWGCPIPIV HCDTCGDVPV PEDQLPVVLP EDVVPDGAGS PLARMPEFYE
CSCPKCGAPA KRETDTMDTF VESSWYYARY ASPHYEGGLV EPNAANHWLP VDQYIGGIEH
AILHLLYARF FHKLMRDEGL VTSNEPFKNL LTQGMVNAET YFRMETSGKK TWINPADVTL
ERDAKAKVIS AKLTSDGLPV EIGGTEKMSK SKKNGIDPQT MIDQYGADTC RLFMMFASPP
DMSLEWSDSG VEGSHRFLRR VWRLAQAHVA QGASTGLDIA ALTDDQKTIR RSIHQAIRQA
SQDIGQNQKF NTAVAQVMTL MNVLEKAPQV TPQDRALLQE GLETVTLLLA PITPHISHEL
WTQLGHNEPV IDAGWPAFDA NALVQDSLQL VIQVNGKLRG HIEMPASASR EEVEAAARIN
ENVLRFTDGL TIRKVIVVPG KLVNIVAS
