ID   SYL_PSEA6               Reviewed;         870 AA.
AC   Q15VK0;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Patl_1566;
OS   Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Pseudoalteromonadaceae; Pseudoalteromonas.
OX   NCBI_TaxID=342610;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T6c / ATCC BAA-1087;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Karls A.C.,
RA   Bartlett D., Higgins B.P., Richardson P.;
RT   "Complete sequence of Pseudoalteromonas atlantica T6c.";
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000388; ABG40088.1; -; Genomic_DNA.
DR   RefSeq; WP_011574402.1; NC_008228.1.
DR   AlphaFoldDB; Q15VK0; -.
DR   SMR; Q15VK0; -.
DR   STRING; 342610.Patl_1566; -.
DR   PRIDE; Q15VK0; -.
DR   EnsemblBacteria; ABG40088; ABG40088; Patl_1566.
DR   KEGG; pat:Patl_1566; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_6; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000001981; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 3.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..870
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000334793"
FT   MOTIF           43..53
FT                   /note="'HIGH' region"
FT   MOTIF           626..630
FT                   /note="'KMSKS' region"
FT   BINDING         629
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   870 AA;  98121 MW;  6FB0DCCA8696AD1F CRC64;
     MAEKIYEPQK IEQEVQALWE TNQTFKATEQ EDKEKFYCLS MFPYPSGRLH MGHVRNYTIG
     DVVSRFQRMN GKNVLQPMGW DAFGLPAENA ALNNNTAPAK WTYQNIDYMK NQLKSLGFGY
     DWDREVATCK KDYYRWEQWF FTRLYEKGLV YKKNSTVNWD PVDHTVLANE QVIDGRGWRS
     GALVEQKEIP QWFIKITDYA EELLTDLEQL EDWPEQVRAM QKNWIGRSEG VDITFNLSEN
     AADISSFDVY TTRPDTLYGV TYVAVAAQHP LALHAAQTRP ELAEFIESCK NTKVAEAELA
     TMEKKGCDTG LFAIHPLTGE KVAVWVANFV LMGYGSGAVM SVPGHDQRDW EFAQKYGLPI
     KQVVEPSADE PQECDLTTAA YTDKGVLVNS AEFNGLAFQE AFDAIAAKLE ALGAGSKKVN
     YRLRDWGVSR QRYWGSPIPM LNLENGESVP VPSDQLPVEL PEDVQMNGVI SPIKADPDWA
     KTEYNGQPAL RETDTFDTFM ESSWYYARYC SAQNQDAMLD PTQANYWLPV DQYVGGIEHA
     ILHLLYSRFF HKLLRDEGLV NSDEPYKRLL CQGMVLADSF FREDESGKKE WFSPADVDTV
     KDEKGRITQA TLKADGKPVE HGGMTKMSKS KNNGIDPQHV IDLYGADTIR VFTMFAAPPE
     QTLEWVDSGV EGANRFIKRV WKLTQDHIDA LDGDTAPAID KTALTGDQKT LRRAVHRTIA
     KVTDDVGRRQ TFNTAVAAIM ELLNSLQRAP QSTDQDKAVL REAIESVVLL LNPIAPHMCH
     ILWHDLGHKN DIETAPWPAV DESALVEDEK LIIVQVNGKV RSKITVAADA SQEEVQALGL
     AQENVQQFVD GKTIRKVIYI AGKLLNIVAN