ID   SYL_PSELT               Reviewed;         815 AA.
AC   A8F8B7;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Tlet_1847;
OS   Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 /
OS   TMO) (Thermotoga lettingae).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Pseudothermotoga.
OX   NCBI_TaxID=416591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Foster B., Bruce D., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K.,
RA   Richardson P.;
RT   "Complete sequence of Thermotoga lettingae TMO.";
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000812; ABV34401.1; -; Genomic_DNA.
DR   RefSeq; WP_012003877.1; NC_009828.1.
DR   AlphaFoldDB; A8F8B7; -.
DR   SMR; A8F8B7; -.
DR   STRING; 416591.Tlet_1847; -.
DR   EnsemblBacteria; ABV34401; ABV34401; Tlet_1847.
DR   KEGG; tle:Tlet_1847; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_0; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000002016; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 2.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 2.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..815
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000071114"
FT   MOTIF           41..51
FT                   /note="'HIGH' region"
FT   MOTIF           576..580
FT                   /note="'KMSKS' region"
FT   BINDING         579
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   815 AA;  94816 MW;  FE81700E65574478 CRC64;
     MPRYEPQEIE SKWQKFWEKE GLFKTPQHSD LPKYYMLVMF PYPSGTLHVG HVKNYVIGDA
     VARYKRMRAY NVLHPFGYDA FGLPAENAAI ERKIHPKDWT LNNINIIRRQ IKKLGISYDW
     NREVITCLES YYKWTEWIFL KLYEAGLAYK KKAAVNWCPK CMTSLANEQV KDGRCERCDT
     PVTIKHLEQW FFKITDYAER LLKDLDKLTG WPEHVKTMQR NWIGESKGAK ISFKVEESEM
     NIEVFTTRPD TLWGVTFMVL APESDLVNHI TLPELKGELE KFLNYVGQQD RHKRGSADVE
     KEGFFTGRYA INPVSGERIP IYVANYVLME YGTGAVMGVP AHDQRDYEFA VKYNLPIKEV
     IKPSEDIPKN RAYDGPGIMV NSGPLNGTVV PEGIERVIQW LEERGIAKRS VQYKLRDWLI
     SRQRYWGAPI PIVYCEKCGM VPVPEKSLPV QLPYDVEFLP TGQSPLMLND EFRKTSCPKC
     GGPALRDADT MDTFVDSSWY FLRYVNPDRD DVPFVKEDVD HWLPVDQYVG GVEHAVLHLL
     YSRFITKVLY DLGYLSFDEP FENLFTQGMI YKDGAKMSKS KGNVVSPDEM IDRYGADTLR
     MYILFMGPPE RDAEWNDAGI EGVYRFIRRA WSLMDQIISL PDNQNQVFAE EEKHLRRKLH
     ISLRKVTQDM EGGFKFNTVV SSLMELVNDA YDYIDNLPQH RWNIKLLKEL AKNFVLMISP
     FAPHFAEELW QRMGYSSSVM KEKWPEYDPD ALVVEEVEIA IQVNGKLRDK VKIPVDATED
     QIKEIALKSE KVRKYISDEP KKIIYVPKRL LNIIF