ID   SYL_PSEMY               Reviewed;         870 AA.
AC   A4XYW8;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Pmen_3786;
OS   Pseudomonas mendocina (strain ymp).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=399739;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ymp;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA   Hersman L., Dubois J., Maurice P., Richardson P.;
RT   "Complete sequence of Pseudomonas mendocina ymp.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABP86534.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000680; ABP86534.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_041772969.1; NC_009439.1.
DR   AlphaFoldDB; A4XYW8; -.
DR   SMR; A4XYW8; -.
DR   STRING; 399739.Pmen_3786; -.
DR   EnsemblBacteria; ABP86534; ABP86534; Pmen_3786.
DR   KEGG; pmy:Pmen_3786; -.
DR   PATRIC; fig|399739.8.peg.3839; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_6; -.
DR   OrthoDB; 32262at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 2.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..870
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000334794"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           629..633
FT                   /note="'KMSKS' region"
FT   BINDING         632
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   870 AA;  96711 MW;  068FCFFC99162D69 CRC64;
     MHEQYSPREI EAAAQSHWDA QKSFVVSEQP GKDTFYCLSM FPYPSGKLHM GHVRNYTIGD
     VIARYQRMQG KNVLQPMGWD AFGMPAENAA MKNQVAPAKW TYENIAYMKS QLKSLGLAID
     WTREVTTCKP DYYRWEQWLF TRLFEKGVIY RKNGTVNWDP VDQTVLANEQ VIDGRGWRSG
     ALIEKREIPM YYFKITAYAD ELLSSLDELD GWPEQVKTMQ RNWIGKSFGA DIVFDYDVAS
     IGIDGQLKVY STRPDTLMGA TYVAVAAEHP LAQRAAESNP AIAAFIAECK AGSVAEADMA
     TMEKKGLATG QFVIHPLTGD KLPVFVANYV LWGYGEGAVM AVPAHDERDF EFANKYDLPI
     KQVYTGEGKD YDASTWQDWY GDKAGLTTIN SGKYDGLDFS AAFDAIVGDL EASAHGARKT
     QFRLRDWGIS RQRYWGCPIP IIHCDSCGDV PVPEDQLPVV LPEDVVPDGA GSPLAKMPEF
     YECTCPKCGA PAKRETDTMD TFVESSWYFA RYASPNYEGG MVDPQAANHW LPVDQYIGGI
     EHAILHLLYA RFFHKLMRDE GLVSSNEPFK NLLTQGMVVA ETYYRTLENG GKDWFNPADV
     EVERDAKAKV IGAKLKSDGL PVEIGGTEKM SKSKNNGVDP QDMIDAYGAD TCRLFMMFAS
     PPDMSCEWSD AGVEGANRFL RRVWRLAHGH VSAGLPGKLD VASLSDEQKA VRRAIHLAIK
     QASNDIGQHH KFNTAIAQVM TLMNVLEKAA TATEQDRALV QEGLEIVTLL LAPITPHISH
     ELWQRLGHAD AVIDAQWPQV DESALVQDSL TLVVQVNGKL RGQIEVPASA SREDVEAAAR
     ANENVLRFTE GLSIRKVIVV PGKLVNIVAN