ID   SYL_PSYCK               Reviewed;         919 AA.
AC   Q1QDA4;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Pcryo_0566;
OS   Psychrobacter cryohalolentis (strain ATCC BAA-1226 / DSM 17306 / VKM B-2378
OS   / K5).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC   Psychrobacter.
OX   NCBI_TaxID=335284;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1226 / DSM 17306 / VKM B-2378 / K5;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D.,
RA   Han C., Tapia R., Sims D.R., Gilna P., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., Richardson P.;
RT   "Complete sequence of chromosome of Psychrobacter cryohalolentis K5.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABE74349.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000323; ABE74349.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_041753373.1; NC_007969.1.
DR   AlphaFoldDB; Q1QDA4; -.
DR   SMR; Q1QDA4; -.
DR   STRING; 335284.Pcryo_0566; -.
DR   KEGG; pcr:Pcryo_0566; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_6; -.
DR   Proteomes; UP000002425; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 2.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..919
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000334798"
FT   MOTIF           83..93
FT                   /note="'HIGH' region"
FT   MOTIF           670..674
FT                   /note="'KMSKS' region"
FT   BINDING         673
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   919 AA;  103257 MW;  892790991B9E9351 CRC64;
     MLWHYKISPH LSVITDSEPT MTNAVTAETA NTNHTNNASS DKTQYQPQLI EAQQQAKWAT
     DKRFEVSNEP SDKPSRYMLS MFPYPSGKLH MGHVRNYTIS DVLSRYYRLK GYEVMQPMGW
     DGFGLPAENA AIANQTPPAK WTFENIDSMR AQLKLLGLSI DWSREFATCS PEYYQWEQWL
     FLQLYKKGLV YKKLATVNWD PVDNTVLANE QVIDGKGWRS GAMVEKRDIP MYYFNITDYA
     DELLDDLDQL EGHWPSEVIT MQRNWIGRSS GMEVHFPYEL AGQSNSLDVF TTRPDTLMGV
     TYVAVAAEHP LAQYASENNK AIADFCALCK KGSVAEADLA KAEKIGMDTG LTVTHPLTGE
     EVPVWVANYV LMSYGSGAVM AVPAHDERDY EFATKYNLPI KQVIDIPAGY FDDVEEGNEN
     CAYTERNTLV NSGEFDGMDF EQAFEAMLAK LEPQELAKKK IQYRLRDWGV SRQRYWGCPI
     PMVNCEHCGT VPVEEQDLPV VLPTDVVPDG RGNPLKNIPE FVNTTCPKCG NPAERETDTF
     DTFVESSWYY ARFASPHDTT NMVNKSAANK WLPVDQYIGG VEHAVMHLLY ARFFHKLMRD
     ENLVSGDEPF ANLMTQGMVL AGTFYRVNAD GSTTYYFTKD IDIDFNERGQ PIKAILKSDG
     QPVTIGKIEK MSKSKNNGVD PQITIDKYGA DTVRLYTLFT APADQTLEWS DDALKGPYNF
     VKKVWRIASE HIQALTDANL SLESLNSDAL NTDSLSKEAK ALRRKTHETI GKIDSDLGKR
     LALNTPVSSL MELANELSNF KASSEQELQV QHEALTDLLI MLSVYAPHIG EYLLEQLGLD
     TVTLNYPMVD ESALVQDMIT MVIQVNGKMR GKMDVAPNSD PEQLKAQARA IEGVAKFLTG
     EIKKEIVVPN KLVNIVVAG