ID   BLO9_KLEPN              Reviewed;         274 AA.
AC   P0A3M3; P22070; Q7WWR7;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 2.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Beta-lactamase OXA-9;
DE            EC=3.5.2.6;
DE   AltName: Full=Oxacillinase;
DE   AltName: Full=Penicillinase;
DE   Flags: Precursor;
GN   Name=bla; Synonyms=oxa9;
OS   Klebsiella pneumoniae.
OG   Plasmid pJMCMW1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TRANSPOSON=Tn1331;
RX   PubMed=1963948; DOI=10.1016/0147-619x(90)90005-w;
RA   Tolmasky M.E.;
RT   "Sequencing and expression of aadA, bla, and tnpR from the multiresistance
RT   transposon Tn1331.";
RL   Plasmid 24:218-226(1990).
RN   [2]
RP   SEQUENCE REVISION TO N-TERMINUS.
RA   Tolmasky M.E.;
RL   Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12384346; DOI=10.1128/aac.46.11.3422-3427.2002;
RA   Sarno R., McGillivary G., Sherratt D.J., Actis L.A., Tolmasky M.E.;
RT   "Complete nucleotide sequence of Klebsiella pneumoniae multiresistance
RT   plasmid pJHCMW1.";
RL   Antimicrob. Agents Chemother. 46:3422-3427(2002).
RN   [4]
RP   FUNCTION.
RX   PubMed=8382826; DOI=10.1006/plas.1993.1004;
RA   Tolmasky M.E., Crosa J.H.;
RT   "Genetic organization of antibiotic resistance genes (aac(6')-Ib, aadA, and
RT   oxa9) in the multiresistance transposon Tn1331.";
RL   Plasmid 29:31-40(1993).
CC   -!- FUNCTION: Oxacillin-hydrolyzing beta-lactamase. Confers resistance to
CC       beta-lactam antibiotics but at a significantly lower level than the TEM
CC       bla gene product. {ECO:0000269|PubMed:8382826}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC         Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC         ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10103};
CC   -!- SIMILARITY: Belongs to the class-D beta-lactamase family.
CC       {ECO:0000305}.
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DR   EMBL; M55547; AAA98406.2; -; Genomic_DNA.
DR   EMBL; AF479774; AAL93143.2; -; Genomic_DNA.
DR   PIR; D37392; D37392.
DR   RefSeq; NP_608309.2; NC_003486.1.
DR   RefSeq; WP_000722315.1; NZ_WWEX01000056.1.
DR   RefSeq; YP_007878590.1; NC_021079.1.
DR   RefSeq; YP_008603397.1; NC_022520.1.
DR   RefSeq; YP_009067787.1; NC_025131.1.
DR   RefSeq; YP_009071734.1; NC_025185.1.
DR   AlphaFoldDB; P0A3M3; -.
DR   SMR; P0A3M3; -.
DR   KEGG; ag:AAA98406; -.
DR   GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR   GO; GO:0017001; P:antibiotic catabolic process; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR002137; Beta-lactam_class-D_AS.
DR   InterPro; IPR001460; PCN-bd_Tpept.
DR   Pfam; PF00905; Transpeptidase; 1.
DR   SUPFAM; SSF56601; SSF56601; 1.
DR   PROSITE; PS00337; BETA_LACTAMASE_D; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Hydrolase; Plasmid; Signal; Transposable element.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..274
FT                   /note="Beta-lactamase OXA-9"
FT                   /id="PRO_0000017036"
FT   ACT_SITE        58
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10103"
FT   BINDING         206..208
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         61
FT                   /note="N6-carboxylysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   274 AA;  30568 MW;  CA96C211CEFF5F2D CRC64;
     MKKILLLHML VFVSATLPIS SVASDEVETL KCTIIADAIT GNTLYETGEC ARRVSPCSSF
     KLPLAIMGFD SGILQSPKSP TWELKPEYNP SPRDRTYKQV YPALWQSDSV VWFSQQLTSR
     LGVDRFTEYV KKFEYGNQDV SGDSGKHNGL TQSWLMSSLT ISPKEQIQFL LRFVAHKLPV
     SEAAYDMAYA TIPQYQAAEG WAVHGKSGSG WLRDNNGKIN ESRPQGWFVG WAEKNGRQVV
     FARLEIGKEK SDIPGGSKAR EDILVELPVL MGNK