SYL_PYRAR
ID SYL_PYRAR Reviewed; 945 AA.
AC A4WHK6;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Pars_0260;
OS Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6).
OC Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC Pyrobaculum.
OX NCBI_TaxID=340102;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700994 / DSM 13514 / JCM 11321 / PZ6;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Cozen A.E.,
RA Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P.;
RT "Complete sequence of Pyrobaculum arsenaticum DSM 13514.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; CP000660; ABP49873.1; -; Genomic_DNA.
DR AlphaFoldDB; A4WHK6; -.
DR SMR; A4WHK6; -.
DR STRING; 340102.Pars_0260; -.
DR PRIDE; A4WHK6; -.
DR EnsemblBacteria; ABP49873; ABP49873; Pars_0260.
DR KEGG; pas:Pars_0260; -.
DR HOGENOM; CLU_004174_0_0_2; -.
DR OMA; AWNMAFQ; -.
DR PhylomeDB; A4WHK6; -.
DR Proteomes; UP000001567; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 1.
DR Gene3D; 3.90.740.10; -; 1.
DR HAMAP; MF_00049_A; Leu_tRNA_synth_A; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR020791; Leu-tRNA-lgase_arc.
DR InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR45794; PTHR45794; 2.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00395; leuS_arch; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..945
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000009403"
FT MOTIF 43..53
FT /note="'HIGH' region"
FT MOTIF 638..642
FT /note="'KMSKS' region"
FT BINDING 641
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 945 AA; 107394 MW; FBF96F309DE85956 CRC64;
MSELARFFIE LGEKWQKRWA EARVYEPAPT LGVPKFFITA AYPYPNGAIH IGHGRTYLIA
DVLARFHRHL GRAVLFPMAF HYTGTPILTI AEAIAAGDET VVEEYMAIYG VPEEEMRKMG
DPLYLARYFH EQSKRAMQKF GLSIDWTREF TTIDPEYQRF IQWQFEKLRK KGLIVRGRHP
VGWCPRHSMP VGAHDTKDDK EPDIGEWTLI YFADRDGLIF PAATLRPETV LGVTNMWINP
EGEYVVAEYD GRKMVLSRDA AYRLSFQGSV KVLREAKGRE FVGREVQNPV TGEWVPIYGA
KFVDPKVGTG VVMSVPAHAP YDYAALRDIG AIRLIPLIKV EGYGEYPAKD VVERMEIKSQ
TDPALEEATK EVYSAEYARG VMREDVVELV GRHLPEPARS MVMAVFKMYF AGRPVREARE
FISKWLAEAG LGGVMYDIMN KPVYCRCGTE IVVKVLEDQW FINYGEPRWK ELAKKLVEEM
TIVPPEAKAQ FFATIDWLDK RACARTRGLG TPLPWSHGWV IESLSDSTIY MAYYAVIKGI
RRHNLRPEQL TEEFWDYVFL GVGTPEEVSA KTGIPAEALR AIREEFEFWY PLDSRNSGKD
LIPNHLTFFI FNHVAIFPRE KWPRQIVANG WVLREGEKMS KSKRNVLPLD KAVEMYGPDP
LRATLAISAE VEQDLDFRHA EAVRNAQQLM SIYTLAQRLA QSAEDREPTW LDRWLLSEVA
LALERVRDAY EKVRVRQAAV ELLYNIKNIF DSYMTAVERP SRLAVEVAKA WAVALEPIAP
HLAEEVWSLL GGEGFVTSAK WPQLKPDPAA LLARRYVDMV VEDVKKIPAY GEGVRRVVLY
INPNFTWVKA ALNNDVKSAI AAGTPPQLAK RLVELVRTLG DEVRSLIAAV ENFDEREALL
SYKNYVEKAL GAPVEVYTAE DPAAPDLGGK KKAALPLKPG IFIER