SYL_PYRHO
ID SYL_PYRHO Reviewed; 967 AA.
AC O58698;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=PH0965;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (3.21 ANGSTROMS).
RG RIKEN structural genomics initiative (RSGI);
RT "The crystal structure of leucyl-tRNA synthetase and tRNA(leucine)
RT complex.";
RL Submitted (SEP-2005) to the PDB data bank.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; BA000001; BAA30062.1; -; Genomic_DNA.
DR PIR; H71087; H71087.
DR RefSeq; WP_010885055.1; NC_000961.1.
DR PDB; 1WKB; X-ray; 2.05 A; A=1-810.
DR PDB; 1WZ2; X-ray; 3.21 A; A/B=1-967.
DR PDBsum; 1WKB; -.
DR PDBsum; 1WZ2; -.
DR AlphaFoldDB; O58698; -.
DR SMR; O58698; -.
DR STRING; 70601.3257379; -.
DR PRIDE; O58698; -.
DR EnsemblBacteria; BAA30062; BAA30062; BAA30062.
DR GeneID; 1443290; -.
DR KEGG; pho:PH0965; -.
DR eggNOG; arCOG00809; Archaea.
DR OMA; AWNMAFQ; -.
DR OrthoDB; 4914at2157; -.
DR BRENDA; 6.1.1.4; 5244.
DR EvolutionaryTrace; O58698; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 1.
DR Gene3D; 3.90.740.10; -; 1.
DR HAMAP; MF_00049_A; Leu_tRNA_synth_A; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR020791; Leu-tRNA-lgase_arc.
DR InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR45794; PTHR45794; 2.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00395; leuS_arch; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..967
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000152140"
FT MOTIF 43..53
FT /note="'HIGH' region"
FT MOTIF 650..654
FT /note="'KMSKS' region"
FT BINDING 653
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
FT HELIX 7..19
FT /evidence="ECO:0007829|PDB:1WKB"
FT TURN 20..23
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 27..29
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 32..34
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 35..40
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 51..70
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 86..95
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 99..107
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 113..117
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 118..120
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 122..139
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 146..148
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 157..172
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 176..186
FT /evidence="ECO:0007829|PDB:1WKB"
FT TURN 187..190
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 195..197
FT /evidence="ECO:0007829|PDB:1WZ2"
FT STRAND 206..215
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 219..221
FT /evidence="ECO:0007829|PDB:1WZ2"
FT STRAND 224..230
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 232..237
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 240..243
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 245..247
FT /evidence="ECO:0007829|PDB:1WZ2"
FT STRAND 249..256
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 259..266
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 267..273
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 279..285
FT /evidence="ECO:0007829|PDB:1WKB"
FT TURN 287..293
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 295..297
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 299..301
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 304..309
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 315..317
FT /evidence="ECO:0007829|PDB:1WZ2"
FT STRAND 322..324
FT /evidence="ECO:0007829|PDB:1WKB"
FT TURN 326..328
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 330..336
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 380..382
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 394..406
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 413..415
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 417..419
FT /evidence="ECO:0007829|PDB:1WZ2"
FT HELIX 420..432
FT /evidence="ECO:0007829|PDB:1WKB"
FT TURN 433..435
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 436..446
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 451..455
FT /evidence="ECO:0007829|PDB:1WZ2"
FT STRAND 457..467
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 472..483
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 485..489
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 490..492
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 493..502
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 509..515
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 521..526
FT /evidence="ECO:0007829|PDB:1WKB"
FT TURN 528..530
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 531..534
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 535..550
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 556..558
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 561..569
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 574..584
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 588..601
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 605..610
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 611..613
FT /evidence="ECO:0007829|PDB:1WKB"
FT TURN 614..616
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 617..628
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 631..633
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 637..641
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 644..646
FT /evidence="ECO:0007829|PDB:1WKB"
FT TURN 653..656
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 661..668
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 670..680
FT /evidence="ECO:0007829|PDB:1WKB"
FT STRAND 687..689
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 691..712
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 724..745
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 749..756
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 758..770
FT /evidence="ECO:0007829|PDB:1WKB"
FT TURN 771..773
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 777..794
FT /evidence="ECO:0007829|PDB:1WKB"
FT TURN 795..797
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 799..808
FT /evidence="ECO:0007829|PDB:1WKB"
FT HELIX 815..817
FT /evidence="ECO:0007829|PDB:1WZ2"
FT HELIX 830..851
FT /evidence="ECO:0007829|PDB:1WZ2"
FT STRAND 858..863
FT /evidence="ECO:0007829|PDB:1WZ2"
FT TURN 867..873
FT /evidence="ECO:0007829|PDB:1WZ2"
FT HELIX 874..877
FT /evidence="ECO:0007829|PDB:1WZ2"
FT TURN 878..880
FT /evidence="ECO:0007829|PDB:1WZ2"
FT STRAND 882..884
FT /evidence="ECO:0007829|PDB:1WZ2"
FT HELIX 885..891
FT /evidence="ECO:0007829|PDB:1WZ2"
FT TURN 894..899
FT /evidence="ECO:0007829|PDB:1WZ2"
FT HELIX 900..902
FT /evidence="ECO:0007829|PDB:1WZ2"
FT HELIX 905..910
FT /evidence="ECO:0007829|PDB:1WZ2"
FT HELIX 922..927
FT /evidence="ECO:0007829|PDB:1WZ2"
FT TURN 928..930
FT /evidence="ECO:0007829|PDB:1WZ2"
FT HELIX 931..938
FT /evidence="ECO:0007829|PDB:1WZ2"
FT STRAND 963..966
FT /evidence="ECO:0007829|PDB:1WZ2"
SQ SEQUENCE 967 AA; 113949 MW; DEFBD287679E9498 CRC64;
MAELNFKAIE EKWQKRWLEA KIFEPNIRDK PKEKKFYITV AFPYLSGHLH VGHARTYTIP
DVIARFKRMQ GYNVLFPMAW HITGSPIVGI AERIKNRDPK TIWIYRDVYK VPEEILWTFE
DPINIVKYFM KAAKETFIRA GFSVDWSREF YTTSLFPPFS KFIEWQFWKL KEKGYIVKGA
HRVRWDPVVG TPLGDHDLME GEDVPILDYI IIKFELRENG EVIYLPAATL RPETVYGVTN
MWVNPNATYV KAKVRRKDKE ETWIVSKEAA YKLSFQDREI EVIEEFKGEK LIGKYVRNPV
SGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHVALEDLK RETEILEKYD IDPRIVENIT
YISLIKLEGY GDFPAVEEVN KLGIKSQKDK EKLEQATKTI YKAEYHKGIF KVPPYEGKPV
QEVKEAIAKE MLEKGIAEIM YEFAEKNVIS RFGNRAVIKI IHDQWFIDYG NPEWKEKARK
ALERMKILPE TRRAQFEAII DWLDKKACAR KIGLGTPLPW DPEWVIESLS DSTIYMAYYT
ISRHINKLRQ EGKLDPEKLT PEFFDYIFLE EFSEDKEKEL EKKTGIPAEI IHEMKEEFEY
WYPLDWRCSG KDLIPNHLTF FIFNHVAIFR EEHWPKGIAV NGFGTLEGQK MSKSKGNVLN
FIDAIEENGA DVVRLYIMSL AEHDSDFDWR RKEVGKLRKQ IERFYELISQ FAEYEVKGNV
ELKDIDRWML HRLNKAIKET TNALEEFRTR TAVQWAFYSI MNDLRWYLRR TEGRDDEAKR
YVLRTLADVW VRLMAPFTPH ICEELWEKLG GEGFVSLAKW PEPVEEWWNE TIEAEEEFIR
SVMEDIKEII EVAKIENAKR AYIYTAEDWK WKVAEVVSEK RDFKSSMEEL MKDSEIRKHG
KEVAKIVQKL IKERTFDVKR INEEKALREA KEFMEKELGI EIIINPTEDK GGKKKQAMPL
KPAIFIE
