ID   SYL_PYRNV               Reviewed;         945 AA.
AC   B1YAS9;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Tneu_0203;
OS   Pyrobaculum neutrophilum (strain DSM 2338 / JCM 9278 / NBRC 100436 /
OS   V24Sta) (Thermoproteus neutrophilus).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC   Pyrobaculum.
OX   NCBI_TaxID=444157;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 2338 / JCM 9278 / NBRC 100436 / V24Sta;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Biddle J.F., Zhang Z., Fitz-Gibbon S.T., Lowe T.M.,
RA   Saltikov C., House C.H., Richardson P.;
RT   "Complete sequence of Thermoproteus neutrophilus V24Sta.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP001014; ACB39158.1; -; Genomic_DNA.
DR   AlphaFoldDB; B1YAS9; -.
DR   SMR; B1YAS9; -.
DR   STRING; 444157.Tneu_0203; -.
DR   EnsemblBacteria; ACB39158; ACB39158; Tneu_0203.
DR   KEGG; tne:Tneu_0203; -.
DR   eggNOG; arCOG00809; Archaea.
DR   HOGENOM; CLU_004174_0_0_2; -.
DR   OMA; AWNMAFQ; -.
DR   Proteomes; UP000001694; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 1.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_A; Leu_tRNA_synth_A; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR020791; Leu-tRNA-lgase_arc.
DR   InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR45794; PTHR45794; 2.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00395; leuS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..945
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000091375"
FT   MOTIF           43..53
FT                   /note="'HIGH' region"
FT   MOTIF           638..642
FT                   /note="'KMSKS' region"
FT   BINDING         641
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   945 AA;  107198 MW;  CC177301AAFCDF38 CRC64;
     MSELSRFFIE LGERWQRRWR EARVFEPEPA PGVPKYFITA AYPYPNGAIH IGHGRTYLVA
     DVMARFQRHL GRSVLFPMGF HYTGTPILTI AEVIAAGDKA VMEEYMELYG VPEEEIKKMG
     DPLYLARYFH GQSKRAMERF GLSIDWTREF TTIDPEYQRF IQWQFEKLRK KGLIVRGRHP
     VGWCPRHSMP VGAHDTKDDK EPDIGQWTLV YFTDSEGLTF PTATLRPETV LGVTNLWINP
     DAEYVVAEFD GRRAVVSRDA AYRLSFQVGV KILREARGRE FVGRMVQNPV TGEWVPVYEA
     RFVDPKVGTG VVMSVPAHAP YDYAALRDLG TVKLIPLIRV EGYGDYPAKE VVERMGIKSQ
     ADPALEDATK EVYSAEYARG VMREDVAERV GAHLEEPARS MLRAVFKMYF AGRPVREARE
     FIARWLTEAR LGGVMYDIMN KPVYCRCGTE IVVKVLEDQW FINYGESRWK EAARELVKEM
     SIVPGEARAQ FLATIDWLDK RACARTRGLG TPLPWSSGWV IESLSDSTIY MAFYTVVKRI
     RQFGIRPEQL TEEFWDFVFL GQGSADEVSK KTGVPVEALK AIREEFEYWY PLDSRNSGKD
     LIPNHLTFFI FNHVAIFPRE KWPRQIVANG WVLREGEKMS KSKRNVLPLD RAVEMYGPDP
     LRATLALAAE VEQDLDFRDA EARRNAQQLM SIYTLAQRLV QGAEERPPTW VDQWLVAEIS
     RVLERAREAY EKVRVRQAAV EVLYNAKAVF DQYLAMVEKP SRQAVEAAKA WAVAMEPLVP
     HLAEELWATL GGAGFAALAP WPKLRAEPAA LLAKRYVDML IEDVKNIPAF GQGAKRVVIY
     VNRSFAWVKA ALAGDVKTVI GAGVPPQQAK KVVDLVKTLG DEMRGLIAAV DHFDELEALR
     SYRNYVEKAL GAPVEIYGAD DPAAPDLGGK KRVALPLKPG IYVEK