ID   BLOT3_BLOTA             Reviewed;         266 AA.
AC   A1KXI1;
DT   31-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Trypsin Blo t 3 {ECO:0000305};
DE            EC=3.4.21.4 {ECO:0000250|UniProtKB:P35030};
DE   AltName: Full=Mite allergen Blo t 3 {ECO:0000303|PubMed:12708986};
DE   AltName: Allergen=Blo t 3 {ECO:0000303|PubMed:12708986, ECO:0000303|PubMed:12752596};
DE   Flags: Precursor;
OS   Blomia tropicalis (Mite).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Acariformes; Sarcoptiformes; Astigmata; Glycyphagoidea; Echimyopodidae;
OC   Blomia.
OX   NCBI_TaxID=40697 {ECO:0000312|EMBL:AAQ24542.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND ALLERGEN.
RX   PubMed=12708986; DOI=10.1034/j.1398-9995.2003.00033.x;
RA   Cheong N., Yang L., Lee B.W., Chua K.Y.;
RT   "Cloning of a group 3 allergen from Blomia tropicalis mites.";
RL   Allergy 58:352-356(2003).
RN   [2] {ECO:0000312|EMBL:AAQ24542.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Chew F.T., Wang W.-L., Shang H.S., Kuay K.T., Lim S.H., Lee B.W.;
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   SUBCELLULAR LOCATION, AND ALLERGEN.
RX   PubMed=12752596; DOI=10.1046/j.1365-2222.2003.01648.x;
RA   Yang L., Cheong N., Wang D.Y., Lee B.W., Kuo I.C., Huang C.H., Chua K.Y.;
RT   "Generation of monoclonal antibodies against Blo t 3 using DNA immunization
RT   with in vivo electroporation.";
RL   Clin. Exp. Allergy 33:663-668(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4;
CC         Evidence={ECO:0000250|UniProtKB:P35030};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12752596}.
CC   -!- ALLERGEN: Causes an allergic reaction in human (PubMed:12708986,
CC       PubMed:12752596). Recombinant protein binds to IgE in 51% of the 45
CC       patients tested allergic to B.tropicalis mites (PubMed:12708986).
CC       Native protein binds to IgE in 57% of the 44 patients tested allergic
CC       to B.tropicalis mites (PubMed:12752596). {ECO:0000269|PubMed:12708986,
CC       ECO:0000269|PubMed:12752596}.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00274, ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY291323; AAQ24542.1; -; mRNA.
DR   AlphaFoldDB; A1KXI1; -.
DR   SMR; A1KXI1; -.
DR   Allergome; 687; Blo t 3.
DR   MEROPS; S01.234; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; ISS:UniProtKB.
DR   GO; GO:0006508; P:proteolysis; ISS:UniProtKB.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR018114; TRYPSIN_HIS.
DR   InterPro; IPR033116; TRYPSIN_SER.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
PE   1: Evidence at protein level;
KW   Allergen; Disulfide bond; Hydrolase; Protease; Secreted; Serine protease;
KW   Signal; Zymogen.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   PROPEP          16..35
FT                   /evidence="ECO:0000250|UniProtKB:P39675, ECO:0000255"
FT                   /id="PRO_0000447699"
FT   CHAIN           36..266
FT                   /note="Trypsin Blo t 3"
FT                   /id="PRO_5012813329"
FT   DOMAIN          36..260
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        75
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        120
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        220
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   SITE            214
FT                   /note="Required for specificity"
FT                   /evidence="ECO:0000305"
FT   DISULFID        60..76
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        187..204
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        216..240
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   CONFLICT        38
FT                   /note="D -> G (in Ref. 1; no nucleotide entry)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        82
FT                   /note="A -> T (in Ref. 1; no nucleotide entry)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        96
FT                   /note="S -> P (in Ref. 1; no nucleotide entry)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        154
FT                   /note="E -> K (in Ref. 1; no nucleotide entry)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   266 AA;  27555 MW;  149251A6FC8CE0DC CRC64;
     MKVLVLFCLV SLAAAGPLKD ALNKAQVDAF YAEGYIVDGS NAADGDAPYQ VSLQRTSHFC
     GGSIIADNYI LTAAHCIQGL SASSLTIRYN TLRHNSGGLT VKASRIIGHE KYDSNTIDND
     IALIQTASKM STGTTNAQAI KLPEQGSDPK ASSEVLITGW GTLSSGASSL PTKLQKVTVP
     IVDRKTCNAN YGAVGADITD NMFCAGILNV GGKDACQGDS GGPVAANGVL VGAVSWGYGC
     AQAKYPGVYT RVGNYISWIK GKGVPV