SYL_RALSO
ID SYL_RALSO Reviewed; 877 AA.
AC Q8XVT3;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=RSc2744;
GN ORFNames=RS00110;
OS Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=267608;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GMI1000;
RX PubMed=11823852; DOI=10.1038/415497a;
RA Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL Nature 415:497-502(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; AL646052; CAD16451.1; -; Genomic_DNA.
DR RefSeq; WP_011002651.1; NC_003295.1.
DR AlphaFoldDB; Q8XVT3; -.
DR SMR; Q8XVT3; -.
DR STRING; 267608.RSc2744; -.
DR EnsemblBacteria; CAD16451; CAD16451; RSc2744.
DR GeneID; 60502246; -.
DR KEGG; rso:RSc2744; -.
DR PATRIC; fig|267608.8.peg.2791; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_4; -.
DR OMA; TFMVLAP; -.
DR Proteomes; UP000001436; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR Gene3D; 3.90.740.10; -; 1.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..877
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000152069"
FT MOTIF 48..58
FT /note="'HIGH' region"
FT MOTIF 636..640
FT /note="'KMSKS' region"
FT BINDING 639
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 877 AA; 97368 MW; 16DAD3713614475F CRC64;
MQDKYSPSEV EQQAQQHWQA QDAYRVTEHA RAADGSDKPK FYACSMLPYP SGKLHMGHVR
NYTINDVMMR QLRMKGYNVL MPMGWDAFGM PAENAALNNG VAPAAWTYDN IAYMKKQMQS
MGLAIDWSRE VATCSPDYYK WNQWLFLKML EKGIAYRKTG TVNWDPIDQT VLANEQVIDG
RGWRSGAVVE KREIPMYYLR ITDYAQELLS DLDPLGWPER VKLMQQNWIG KSEGVRFAFP
HGIPGDDGKV IGDGKLYVFT TRADTIMGVT FCAVAAEHPI AAHAAQGNPA LAAFIDECKH
GSVMEADMAT MEKKGMPTGL TVTHPLTGEA VPVWVGNYVL MTYGDGAVMG VPAHDERDFA
FANKYGLAIK QVIDVKGQPF GTEAWQEWYA DKERGVLVHS GKYDGLDYRQ AVDAVAADLA
AQGLGEKKTT WRLRDWGISR QRYWGTPIPL IHCDSCGVVP VPEQDLPVRL PEDLVPDGSG
NPLAKDARFL ECTCPSCGKP ARRETDTMDT FIDSCWYYMR YTCPDGATMV DGRNDYWMPM
DQYIGGIEHA ILHLLYARFW TKVMRDLGLV KFDEPFTKLL TQGMVLNETY YREDAAGKKQ
WINPADVDVQ TDDRGRPIGA TLKADGQPVV IGGVEKMSKS KNNGIDPQAL IDQYGADTAR
LFTMFAAPPE QQLEWSDAGV EGASRFLRRA WNFGVAHAES IRAGHGNGVV NGATDADRAL
RRELHTVLKQ ANYDYERLQY NTVVSAAMKM LNALEGAKDA GADARREGLG ILLRVLYPVV
PHITHVLWQE LGYAGAYGGL LDAPWPQVDE GALVQSEIEL VLQVNGKVRG SIVVPADADR
AAIEAIAAKD EAVHRFAEGK PPKKIIVVPG RLVNVVA