ID   SYL_RHIWR               Reviewed;         835 AA.
AC   A5VA89;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Swit_2852;
OS   Rhizorhabdus wittichii (strain DSM 6014 / CCUG 31198 / JCM 15750 / NBRC
OS   105917 / EY 4224 / RW1) (Sphingomonas wittichii).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Rhizorhabdus.
OX   NCBI_TaxID=392499;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6014 / CCUG 31198 / JCM 15750 / NBRC 105917 / EY 4224 / RW1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C.,
RA   Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Kim E., Halden R.U., Miller T.R., Salzberg S.L., Eisen J.A.,
RA   Richardson P.;
RT   "Complete sequence of chromosome of Sphingomonas wittichii RW1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000699; ABQ69205.1; -; Genomic_DNA.
DR   RefSeq; WP_012049055.1; NC_009511.1.
DR   AlphaFoldDB; A5VA89; -.
DR   SMR; A5VA89; -.
DR   STRING; 392499.Swit_2852; -.
DR   EnsemblBacteria; ABQ69205; ABQ69205; Swit_2852.
DR   KEGG; swi:Swit_2852; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_5; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000001989; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 3.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..835
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000334823"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           612..616
FT                   /note="'KMSKS' region"
FT   BINDING         615
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   835 AA;  92289 MW;  D328A1D3BF136638 CRC64;
     MSARFNHLKA DAHWQGVWES QGTFQARDDS PKPKSYVLEM FPYPSGRIHM GHVRNYTMGD
     VLARYRRMKG FEVLHPMGWD AFGMPAENAA MEKKVHPGDW TRANIAAMRD QLKRIGFALD
     WSRELATCEP DYYGQEQALF LDLYAAGLVY RKESAVNWDP VDMTVLANEQ VIDGRGWRSG
     ALVERRKLNQ WFLKITDFAD DLLDGLDTLD QWPEKVRTMQ ENWIGKSQGM RFTFVLDAPA
     GDLTSFDVFT TRPDTMFGAS FAAIAADHPI AQALSAGNPA LQAFIADCKR TGTAAAEIET
     AEKKGYDTGL SVVHPLDPDW KLPLFVANFV LMDYGTGAVF GCPAHDQRDL DFARKYALPV
     KRVVAPSPEE AAAPIGDEAY VGPGRIVNSA FLDGLSVEEA KAAVIARAEA GGWGQGTTVW
     RLRDWGVSRQ RYWGTPIPII HCEGCGAVPV PHDQLPVVLP EDVAFDIPGN PLDRHPTWKH
     VDCPSCGKPA RRETDTLDTF VDSSWYFIRF ASQPSDKPFD RAVAESWMPV GQYIGGVEHA
     ILHLLYARFW TRALERIGRI GVKEPFTGLF TQGMVTHETY KDPAGQWLSP EEVSDGIVVA
     TGAPATVGRI EKMSKSKKNV VDPAPIVEQY GADAVRWFML SDSPPERDLE WTESGIEGCW
     RFVNRLWRIT DVSEAGDGED RELDRKLHKA IAGIAADIEA LGFNRAVAKI HELSNAIEKA
     APSASRAAAA RALVRLVAPM VPHLAEEAWA RLGEQGLVAD AAWPAHDPAL LVDDEVTIAV
     QVNGKLRDTL TAPKGAPKDA LERMALESEK ITRLLEGAAP RKVIVVPDRL VNIVA