ID   SYL_RHOPA               Reviewed;         876 AA.
AC   Q6ND22;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=RPA0287;
OS   Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Rhodopseudomonas.
OX   NCBI_TaxID=258594;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-98 / CGA009;
RX   PubMed=14704707; DOI=10.1038/nbt923;
RA   Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L.,
RA   Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R.,
RA   Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C.,
RA   Harrison F.H., Gibson J., Harwood C.S.;
RT   "Complete genome sequence of the metabolically versatile photosynthetic
RT   bacterium Rhodopseudomonas palustris.";
RL   Nat. Biotechnol. 22:55-61(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; BX572593; CAE25731.1; -; Genomic_DNA.
DR   RefSeq; WP_011155855.1; NC_005296.1.
DR   AlphaFoldDB; Q6ND22; -.
DR   SMR; Q6ND22; -.
DR   STRING; 258594.RPA0287; -.
DR   PRIDE; Q6ND22; -.
DR   EnsemblBacteria; CAE25731; CAE25731; RPA0287.
DR   GeneID; 66891297; -.
DR   KEGG; rpa:RPA0287; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_5; -.
DR   OMA; TFMVLAP; -.
DR   PhylomeDB; Q6ND22; -.
DR   BioCyc; RPAL258594:TX73_RS01490-MON; -.
DR   Proteomes; UP000001426; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 2.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..876
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000152073"
FT   MOTIF           43..53
FT                   /note="'HIGH' region"
FT   MOTIF           632..636
FT                   /note="'KMSKS' region"
FT   BINDING         635
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   876 AA;  97725 MW;  8FDD1478FE408442 CRC64;
     MSNERYNARE SEPKWQAKWD EAKIFATRND DLRKKYYVLE MFPYPSGRIH MGHVRNYTMG
     DVVARTMRAR GYNVLHPMGW DAFGLPAENA AIERKVAPKA WTYDNIAAMK KQLQTMGLSL
     DWAREFATCD PSYYKHQQKM FLDFLKVGLV EREKRKLNWD PVDMTVLANE QVIDGRGWRS
     GAVVELREMN QWVFKITKYA QELLDALDTL DRWPDKVRLM QRNWIGRSEG LMVRFALDSA
     TTPAGETELK IFTTRPDTLF GAKFMAIAAD HPLAQAAAAK APKVAAFIDD CKKRGTAQAE
     IDTAEKQGID TGIRAVHPFD PSWKLPVYVA NFVLMEYGTG AIFGCPAHDQ RDLDFVNKYQ
     LGNTPVVCPE GQDPASFVIT DTAYDGEGRM INSRFLDGKT IAEAKEEVAK RLETEQLAGA
     PVGARKVNFR LRDWGISRQR YWGCPIPIIH CPTCDVVPVP DADLPVVLPE DVSFDKPGNA
     LDHHPTWKHV TCPKCGGKAV RETDTMDTFV DSSWYFARFT DPWNTEAPTT PDVVNRMMPV
     DQYIGGVEHA ILHLLYSRFF TRAMKAAGHI DIQHDEPFAG LFTQGMVVHE TYRKADGHFA
     SPAEISITVE GDTRRATLLD GGSPVEIGPI EKMSKSKRNT VDPDDIIGTY GADTARWFML
     SDSPPDRDVI WSEEGVKGAS RFVQRLWRMV NDAAPIAASA PAERPASFGA DALTLRKAAH
     GALDKVLSGI ERLAFNVSLA HIREFSNTLG DALARSQTPS PDLAWAIRES TVILVQLFHP
     MMPHLAEECW TVLGQTGLVS EALWPPIEQD LLVEDSITLP VQVNGKKRGD VTVPRDAPTS
     EIEAAVLALD TVKQALGGKP VRKVIVVPQR IVNVVG