BLP4_PSEAI
ID BLP4_PSEAI Reviewed; 288 AA.
AC P16897;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Beta-lactamase PSE-4;
DE EC=3.5.2.6;
DE AltName: Full=Beta-lactamase CARB-1;
DE AltName: Full=Carbenicillinase 1;
DE Flags: Precursor;
GN Name=pse4; Synonyms=carB1;
OS Pseudomonas aeruginosa.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Dalgleish; TRANSPOSON=Tn1405;
RX PubMed=2295609; DOI=10.1016/s0021-9258(19)40181-6;
RA Boissinot M., Levesque R.C.;
RT "Nucleotide sequence of the PSE-4 carbenicillinase gene and correlations
RT with the Staphylococcus aureus PC1 beta-lactamase crystal structure.";
RL J. Biol. Chem. 265:1225-1230(1990).
CC -!- FUNCTION: Hydrolyzes both carbenicillin and oxacillin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10101};
CC -!- SIMILARITY: Belongs to the class-A beta-lactamase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA25979.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; J05162; AAA25979.1; ALT_INIT; Genomic_DNA.
DR PIR; A35001; A35001.
DR RefSeq; WP_063857835.1; NZ_LLOM01000076.1.
DR PDB; 1G68; X-ray; 1.95 A; A=18-288.
DR PDB; 1G6A; X-ray; 1.75 A; A=18-288.
DR PDB; 4ID4; X-ray; 1.05 A; A=145-185.
DR PDB; 4R4R; X-ray; 1.20 A; A=145-185.
DR PDBsum; 1G68; -.
DR PDBsum; 1G6A; -.
DR PDBsum; 4ID4; -.
DR PDBsum; 4R4R; -.
DR AlphaFoldDB; P16897; -.
DR BMRB; P16897; -.
DR SMR; P16897; -.
DR ChEMBL; CHEMBL1744489; -.
DR BRENDA; 3.5.2.6; 5087.
DR SABIO-RK; P16897; -.
DR EvolutionaryTrace; P16897; -.
DR GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR GO; GO:0030655; P:beta-lactam antibiotic catabolic process; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 3.40.710.10; -; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR045155; Beta-lactam_cat.
DR InterPro; IPR000871; Beta-lactam_class-A.
DR InterPro; IPR023650; Beta-lactam_class-A_AS.
DR PANTHER; PTHR35333; PTHR35333; 1.
DR Pfam; PF13354; Beta-lactamase2; 1.
DR PRINTS; PR00118; BLACTAMASEA.
DR SUPFAM; SSF56601; SSF56601; 1.
DR PROSITE; PS00146; BETA_LACTAMASE_A; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic resistance; Disulfide bond; Hydrolase; Signal;
KW Transposable element.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..288
FT /note="Beta-lactamase PSE-4"
FT /id="PRO_0000017047"
FT ACT_SITE 65
FT /note="Acyl-ester intermediate"
FT BINDING 229..231
FT /ligand="substrate"
FT DISULFID 72..118
FT /evidence="ECO:0000250"
FT HELIX 23..35
FT /evidence="ECO:0007829|PDB:1G6A"
FT STRAND 39..46
FT /evidence="ECO:0007829|PDB:1G6A"
FT TURN 47..50
FT /evidence="ECO:0007829|PDB:1G6A"
FT STRAND 51..56
FT /evidence="ECO:0007829|PDB:1G6A"
FT HELIX 64..66
FT /evidence="ECO:0007829|PDB:4R4R"
FT HELIX 68..80
FT /evidence="ECO:0007829|PDB:1G6A"
FT STRAND 89..91
FT /evidence="ECO:0007829|PDB:1G6A"
FT HELIX 94..96
FT /evidence="ECO:0007829|PDB:1G6A"
FT HELIX 104..106
FT /evidence="ECO:0007829|PDB:1G6A"
FT STRAND 110..113
FT /evidence="ECO:0007829|PDB:1G6A"
FT HELIX 114..124
FT /evidence="ECO:0007829|PDB:1G6A"
FT HELIX 127..136
FT /evidence="ECO:0007829|PDB:1G6A"
FT HELIX 145..149
FT /evidence="ECO:0007829|PDB:4ID4"
FT HELIX 163..165
FT /evidence="ECO:0007829|PDB:4ID4"
FT HELIX 178..185
FT /evidence="ECO:0007829|PDB:4ID4"
FT STRAND 191..194
FT /evidence="ECO:0007829|PDB:1G6A"
FT HELIX 196..207
FT /evidence="ECO:0007829|PDB:1G6A"
FT TURN 213..215
FT /evidence="ECO:0007829|PDB:1G6A"
FT HELIX 216..219
FT /evidence="ECO:0007829|PDB:1G6A"
FT STRAND 225..232
FT /evidence="ECO:0007829|PDB:1G6A"
FT HELIX 234..236
FT /evidence="ECO:0007829|PDB:1G6A"
FT STRAND 238..246
FT /evidence="ECO:0007829|PDB:1G6A"
FT STRAND 249..260
FT /evidence="ECO:0007829|PDB:1G6A"
FT HELIX 265..284
FT /evidence="ECO:0007829|PDB:1G6A"
SQ SEQUENCE 288 AA; 31406 MW; 0E618F058720F3A5 CRC64;
MKFLLAFSLL IPSVVFASSS KFQQVEQDVK AIEVSLSARI GVSVLDTQNG EYWDYNGNQR
FPLTSTFKTI ACAKLLYDAE QGKVNPNSTV EIKKADLVTY SPVIEKQVGQ AITLDDACFA
TMTTSDNTAA NIILSAVGGP KGVTDFLRQI GDKETRLDRI EPDLNEGKLG DLRDTTTPKA
IASTLNKFLF GSALSEMNQK KLESWMVNNQ VTGNLLRSVL PAGWNIADRS GAGGFGARSI
TAVVWSEHQA PIIVSIYLAQ TQASMEERND AIVKIGHSIF DVYTSQSR
