ID   SYL_RIPO1               Reviewed;         856 AA.
AC   B7JYA9;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   OrderedLocusNames=PCC8801_3236;
OS   Rippkaea orientalis (strain PCC 8801) (Cyanothece sp. (strain PCC 8801)).
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Aphanothecaceae; Rippkaea; Rippkaea orientalis.
OX   NCBI_TaxID=41431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 8801;
RX   PubMed=21972240; DOI=10.1128/mbio.00214-11;
RA   Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H.,
RA   Sherman L.A., Pakrasi H.B.;
RT   "Novel metabolic attributes of the genus Cyanothece, comprising a group of
RT   unicellular nitrogen-fixing Cyanobacteria.";
RL   MBio 2:E214-E214(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP001287; ACK67211.1; -; Genomic_DNA.
DR   RefSeq; WP_012596472.1; NC_011726.1.
DR   AlphaFoldDB; B7JYA9; -.
DR   SMR; B7JYA9; -.
DR   STRING; 41431.PCC8801_3236; -.
DR   EnsemblBacteria; ACK67211; ACK67211; PCC8801_3236.
DR   KEGG; cyp:PCC8801_3236; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_3; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000008204; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 2.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..856
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000199191"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           617..621
FT                   /note="'KMSKS' region"
FT   BINDING         620
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   856 AA;  97318 MW;  C5B2469C542BE1FE CRC64;
     MESQYNAAAI EQKWQQDWVK QGLDKTPENS NKPKFYALSM FPYPSGNLHM GHVRNYVITD
     VIARLKRLQG YRVLHPMGWD AFGLPAENAA IDRGIPPAEW TYKNMSQMKQ QLQTLGLSID
     WEREVATCAP EYYKWTQWLF LQLYQAGLAY QKEAAVNWDP IDQTVLANEQ VDSEGYSWRS
     GAKVERKLLR QWFLKITDYA EQLLTDLDKL PGWPDRVKLM QENWIGKSVG AYLEFPIKGS
     EEKIAVFTTR PDTVYGVTYV VLAPEHPLTL KVTTPEQKAA VDAFIEEVSN ESEIERTAED
     KPKRGILTGG TAINPFNGQE IPILIADYVL YEYGTGAVMG VPAHDTRDFK FAKEKQLPIK
     VVIIPENSDN TNPPLTEAYT DPGIMVNSGI FDGIQSTEGK TAIIEYAEKE GYGKARIQYR
     LRDWLISRQR YWGCPIPVVH CPSCGTVAVP DADLPVKLPE NVEFTGRDAS PLAKLEDWIN
     VPCPSCGEPA KRETDTMDTF IDSSWYYLRY TDANNNQEAF NLEKANDWMA IDQYVGGIEH
     AILHLLYSRF FTKALRDRGL VNIDEPFKRL LTQGMVQGLT YKNPQTGKYV PSENVIFQDD
     HYRDKETGEI LSFFYEKMSK SKYNGVDPKQ VLGKYGADTA RMFILFKAPP EKDLEWDDAD
     VEGQFRFLNR VWRLVANYLE NKPKTVKKAS ELTKEDKDLR RAIHTAIKEI SEDLEGDYQF
     NTAVSELMKL SNALTDAKCL ESGVYQEGIE TLLILLAPFA PHIAEELWHN LGYEKSIHLQ
     SWPTVNPDAL VVDEITLVIQ IMGKTRGTIQ VSATATKDEL EKLARESEVG QRYLDGQDVK
     KVIVVPGKLV NFVIAK