ID   SYL_RUEST               Reviewed;         857 AA.
AC   Q1GKN8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2006, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=TM1040_0045;
OS   Ruegeria sp. (strain TM1040) (Silicibacter sp.).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Roseobacteraceae; Ruegeria; unclassified Ruegeria.
OX   NCBI_TaxID=292414;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TM1040;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Brettin T., Bruce D., Han C., Tapia R., Goodwin L., Thompson L.S.,
RA   Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA   Belas R., Moran M.A., Buchan A., Gonzalez J.M., Schell M.A., Sun F.,
RA   Richardson P.;
RT   "Complete sequence of chromosome of Silicibacter sp. TM1040.";
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000377; ABF62778.1; -; Genomic_DNA.
DR   RefSeq; WP_011537416.1; NC_008044.1.
DR   AlphaFoldDB; Q1GKN8; -.
DR   SMR; Q1GKN8; -.
DR   STRING; 292414.TM1040_0045; -.
DR   EnsemblBacteria; ABF62778; ABF62778; TM1040_0045.
DR   KEGG; sit:TM1040_0045; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_5; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000000636; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..857
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009433"
FT   MOTIF           41..51
FT                   /note="'HIGH' region"
FT   MOTIF           628..632
FT                   /note="'KMSKS' region"
FT   BINDING         631
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   857 AA;  95857 MW;  67FE4D9302BC6DA8 CRC64;
     MSRYEASDIE ARWQKAWEEA AIFQAKEDHS KPKYYVLEMF PYPSGKLHMG HVRNYTMGDV
     IARYKLSTGH NVLHPMGFDA FGMPAENAAM ASGGHPKDWT YSNIDTMVEQ MKPLGLSLDW
     SRMFATCDPE YYGQQQALFL DFLEKGLVYR KNAVVNWDPV DMTVLANEQV ENGRGWRSGA
     LVERRELTQW FFKISDYSDE LLEALDGLEN WPAKVRLMQE NWIGKSRGLQ FSFERTDGEE
     AIEVYTTRPD TLMGASFVGI SPDHPIAKKL EAESQEVADF VAECRKGGTT EEAIETAEKL
     GYDTGLRVKH PLDPNWELPV WIANFILMDY GTGAIFACPA HDQRDFEFAT KYNLPIIPVL
     EPVEGAGELT EAYVPTKAEK VRYVRGFAGD ELQTGEEGVN TAVDKAEAEG WGQGVTKFRL
     RDWGLSRQRY WGCPIPVVHC PDCGVVPEKK ENLPIALPYD EDGKAIDFSV PGNPLDRHPS
     WRNCACPNCG KPAQRETDTM DTFVDSSWYF ARFTAPRAET PTDLDAANYW MNVDQYIGGI
     EHAILHLLYS RFFARAMQIC GHLPESAKEP FDALFTQGMV THAIYENAER KDDNGRAIYH
     YPSEVEERDG GVFVKETGER IKVIPSAKMS KSKNNVVDPL AIISTYGADT ARWFVLSDSP
     PERDVEWTAS GAEAAYKHLN RVWNLCDRIG EMDPDAKGEG DEELLREMHK AIRDVTLGVE
     SFGFNAAIAK LYGFTNTLSK SKASAAVQKQ AIKTLAQLMS PMTPHLAEDI WAHQGGEGLI
     ANAAWPVADE SMLVEDSVTL PIQVNGKRRG EITVAKDLDK AEVEKLALAH EAVQRVLEGA
     APKKVIVVPG RIVNVVA